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Phytochemical Analysis, Antioxidant and Antimicrobial Screening of Seriphidium Oliverianum Plant Extracts
- Source :
- Dose-Response
- Publication Year :
- 2021
- Publisher :
- SAGE Publications, 2021.
-
Abstract
- The aim of this study was to investigate the phytochemicals using reverse-phase high pressure liquid chromatography (RP-HPLC), antioxidant, antifungal and antibacterial activities of Seriphidium oliverianum stem extracts. The extraction was carried out by conventional shaking process (CSP) and ultrasonic assisted process (UAP). The highest total phenolic contents (97.85 ± 0.735 mg gallic acid equivalent (GAE)/g sample) and flavonoid contents (188.15 ± 0.53 mg catechin equivalent (CE)/g sample) were found in methanol extract obtained by CSP. Antioxidant activity was investigated using DPPH° scavenging assay and reducing power assay. Methanol extract using UAP showed the highest DPPH° scavenging activity (79.95% ± 1.80%) followed by methanol and butanol extracts obtained through CSP. Moreover, methanol extracts using CSP showed highest reducing activity (1.032 ± 0.0205 absorbance). In-vitro antimicrobial activity was studied using most common infection causing fungal and bacterial strains. Anti-fungal activity of methanol extract using CSP showed the highest zone of inhibition (10.5 mm) against F. avenaceum fungal strain, while aqueous extracts obtained through showed the highest antibacterial activity (22 ± 1.32 mm zone of inhibition) against S. aureus. The results showed that the methanol stem extract of S. oliverianum is a valued candidate for further screening and could be processed for in-vivo infection induced animal trials.
- Subjects :
- Health, Toxicology and Mutagenesis
Flavonoid
Seriphidium oliverianum
Toxicology
030226 pharmacology & pharmacy
01 natural sciences
High-performance liquid chromatography
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Agar diffusion test
Food science
Gallic acid
polyphenols
chemistry.chemical_classification
antimicrobial activity
Chemical Health and Safety
010405 organic chemistry
Public Health, Environmental and Occupational Health
Catechin
solvent extraction
0104 chemical sciences
antioxidants
chemistry
Phytochemical
Polyphenol
RP-HPLC
Original Article
Antibacterial activity
Subjects
Details
- ISSN :
- 15593258
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Dose-Response
- Accession number :
- edsair.doi.dedup.....4feab7c7922211746a816fa6bd121392