Additional file 1 of Tonicity-responsive enhancer-binding protein promotes diabetic neuroinflammation and cognitive impairment via upregulation of lipocalin-2

Additional file 1: Fig. S1. Increased weight and HAFLD in HFD-fed mice with or without STZ. Fig. S2. Insulin resistance in HFD-fed mice with or without STZ. Fig. S3. TonEBP and LCN2 expression in the hippocampus of HFD-fed mice with or without STZ. Fig. S4. TonEBP (+ / ���) mice show ameliorated HFD/STZ-induced hepatic steatosis and eWAT inflammation. Fig. S5. TonEBP-and LCN2-positive neutrophils are observed in epididymal white adipose tissue of HFD/STZ-induced diabetic mice. Fig. S6. TonEBP-and LCN2-positive neurons are observed in hippocampal CA1 region of HFD/STZ-induced diabetic mice. Fig. S7. The TonEBP binding site in the mouse LCN2 promoter. Table S1. List of primary antibodies. Table S2. List of qRT-PCR primers. Table S3. List of qRT-PCR primer for ChIP assay. Table S4. Clinical characteristics of normal subjects (CTL) and type 2 diabetic patients (DM) with or without mild cognitive impairment (MCI).