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Glycation-assisted synthesized gold nanoparticles inhibit growth of bone cancer cells
- Source :
- Colloids and Surfaces B: Biointerfaces. 117:473-479
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- This study presents a novel approach to synthesize glycogenic gold nanoparticles (glycogenic GNps) capped with glycated products (Schiff's base, Heyns products, fructosylamine etc.). These glycogenic GNps have been found to be active against human osteosarcoma cell line (Saos-2) with an IC 50 of 0.187 mM, while the normal human embryonic lung cell line (L-132) remained unaffected up to 1 mM concentration. The size of glycogenic GNps can also be controlled by varying the time of incubation of gold solution. Glycation reactions involving a combination of fructose and HSA (Human Serum Albumin) were found to be effective in the reduction of gold to glycogenic GNps whereas glucose in combination with HSA did not result in the reduction of gold. The progress of the reaction was followed using UV–visible spectroscopy and NBT (Nitroblue tetrazolium) assay. The glycogenic GNps were found to be spherical in shape with an average size of 24.3 nm, in a stable emulsion. These GNps were characterized using UV–visible spectroscopy, zeta potential analysis, transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
- Subjects :
- Glycosylation
Indoles
Cell Survival
Scanning electron microscope
Static Electricity
Metal Nanoparticles
Antineoplastic Agents
Apoptosis
Fructose
chemistry.chemical_compound
Colloid and Surface Chemistry
Glycation
Cell Line, Tumor
medicine
Humans
Physical and Theoretical Chemistry
Cell Shape
Serum Albumin
Cell Proliferation
Osteosarcoma
Surfaces and Interfaces
General Medicine
Human serum albumin
Glucose
chemistry
Biochemistry
Colloidal gold
Transmission electron microscopy
Cell culture
Emulsion
Spectrophotometry, Ultraviolet
Gold
Biotechnology
Nuclear chemistry
medicine.drug
Subjects
Details
- ISSN :
- 09277765
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Colloids and Surfaces B: Biointerfaces
- Accession number :
- edsair.doi.dedup.....5044b7a208b3817079b364d630f30275