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A 5-methylcytosine DNA glycosylase/lyase demethylates the retrotransposon Tos17 and promotes its transposition in rice
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 108(37)
- Publication Year :
- 2011
-
Abstract
- DNA 5-methylcytosine (5-meC) is an important epigenetic mark for transcriptional gene silencing in many eukaryotes. In Arabidopsis , 5-meC DNA glycosylase/lyases actively remove 5-meC to counteract transcriptional gene silencing in a locus-specific manner, and have been suggested to maintain the expression of transposons. However, it is unclear whether plant DNA demethylases can promote the transposition of transposons. Here we report the functional characterization of the DNA glycosylase/lyase DNG701 in rice. DNG701 encodes a large (1,812 amino acid residues) DNA glycosylase domain protein. Recombinant DNG701 protein showed 5-meC DNA glycosylase and lyase activities in vitro. Knockout or knockdown of DNG701 in rice plants led to DNA hypermethylation and reduced expression of the retrotransposon Tos17 . Tos17 showed less transposition in calli derived from dng701 knockout mutant seeds compared with that in wild-type calli. Overexpression of DNG701 in both rice calli and transgenic plants substantially reduced DNA methylation levels of Tos17 and enhanced its expression. The overexpression also led to more frequent transposition of Tos17 in calli. Our results demonstrate that rice DNG701 is a 5-meC DNA glycosylase/lyase responsible for the demethylation of Tos17 and this DNA demethylase plays a critical role in promoting Tos17 transposition in rice calli.
- Subjects :
- Transposable element
DNA, Plant
Retroelements
Lyases
Retrotransposon
Biology
law.invention
DNA Glycosylases
chemistry.chemical_compound
Gene Knockout Techniques
MUTYH
law
Gene Expression Regulation, Plant
Multidisciplinary
fungi
food and beverages
Oryza
DNA Methylation
Biological Sciences
Plants, Genetically Modified
Molecular biology
chemistry
DNA glycosylase
Gene Knockdown Techniques
DNA methylation
Recombinant DNA
biology.protein
5-Methylcytosine
Demethylase
DNA
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 108
- Issue :
- 37
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....5050c64a928509451be3c7dfc3bd1086