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Cannabinoid Receptor Agonist WIN55, 212-2 Adjusts Lipid Metabolism in a Rat Model of Cardiac Arrest
- Source :
- Ther Hypothermia Temp Manag
- Publication Year :
- 2020
- Publisher :
- Mary Ann Liebert, Inc., publishers, 2020.
-
Abstract
- The objective of this study was to investigate the effects of pharmacologically induced hypothermia with WIN55, 212-2 (WIN)on postresuscitation myocardial function, microcirculation, and metabolism-specific lipids in a rat cardiac arrest (CA) model. Ventricular fibrillation was electrically induced and untreated for 6 minutes in 24 Sprague-Dawley rats weighing 450–550 g. Cardiopulmonary resuscitation including chest compression and mechanical ventilation was then initiated and continued for 8 minutes, followed by defibrillation. At 5 minutes after restoration of spontaneous circulation (ROSC), animals were randomized into four groups: (1) normothermia with vehicle (NT); (2) physical hypothermia with vehicle (PH); (3) WIN55, 212-2 with normothermia (WN); and (4) WIN55, 212-2 with hypothermia (WH). For groups of WN and WH, WIN was administered by continuous intravenous infusion with a syringe pump for 4 hours. PH started at 5 minutes after resuscitation. NT maintained core temperature at 37°C ± 0.2°C with the aid of a heating blanket. Hypothermia groups maintained temperature at 33°C ± 0.5°C for 4 hours after ROSC. There was a significant improvement in myocardial function as measured by ejection fraction, cardiac output, and myocardial performance index in animals treated with WH and PH beginning at 1 hour after start of infusion. In the WH and PH groups, buccal microcirculation was significantly improved compared with NT and WN. Plasma at pre-CA and ROSC 4 hours was harvested for lipid metabolism. The WH group appeared to be closer to baseline than the other groups in lipid metabolism. lysophosphatidylcholine (LPC) 18:2, free fatty acid (FFA) 22:6, and ceramide (CER) (24:0) changed significantly among the lipidomic data compared with NT (p
- Subjects :
- Agonist
Cannabinoid receptor
medicine.drug_class
medicine.medical_treatment
Pharmacology
Critical Care and Intensive Care Medicine
Microcirculation
Rats, Sprague-Dawley
Hypothermia, Induced
medicine
Animals
Cardiopulmonary resuscitation
Cannabinoid Receptor Agonists
business.industry
Lipid metabolism
Metabolism
Original Articles
Hypothermia
Lipid Metabolism
Cardiopulmonary Resuscitation
Heart Arrest
Rats
Disease Models, Animal
Anesthesiology and Pain Medicine
Ventricular Fibrillation
Shivering
medicine.symptom
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Ther Hypothermia Temp Manag
- Accession number :
- edsair.doi.dedup.....506bc687abb96af6160151cf18d68d9c