Back to Search
Start Over
PROTEIN-PHOSPHORYLATION AT TYROSINE RESIDUES IN V-ABL TRANSFORMED MOUSE LYMPHOCYTES AND FIBROBLASTS
- Publication Year :
- 1986
- Publisher :
- JOHN WILEY & SONS INC, 1986.
-
Abstract
- Phosphotyrosine antibodies were employed to immunodecorate and immunoprecipitate proteins phosphorylated at tyrosine residues in cells transformed by Abelson murine leukemia virus (A-MuLV). In pre-B and pre-T lymphoma cells transformed by A-MuLV, the major phosphotyrosine-containing protein has an MW of 160 kDa and shares immunologically detectable sequences with the v-abl oncogene product. Moreover, two different proteins of approximately 100 and 68 kDa, heavily phosphorylated at tyrosine, were identified. Lack of immunological cross-reactivity with viral products and phosphopeptide mapping showed that the 100 and 68 kDa proteins are coded by cellular genes. Phosphoproteins were undetectable in control resting lymphocytes. The 68 and the 100 kDa proteins were phosphorylated to different extents in proliferating lymphocytes, either stimulated by the growth factor IL-2, or transformed by M-MuLV (lacking the oncogene coded kinase). In fibroblasts transformed by A-MuLV, phosphotyrosine antibodies identified 2 proteins of 120 and 70 kDa. By immunological cross-reaction and by phosphopeptide mapping, the first was identified as a 120 kDa form of the v-abl coded kinase. The 70 kDa protein is coded by a cellular gene, is not structurally related to the 120 kDa v-abl kinase, and is different from any phosphotyrosine-containing protein detected in A-MuLV-transformed lymphocytes. These data show that, upon v-abl-induced transformation, phosphorylation at tyrosine takes place also on proteins other than the 160 or 120-kDa oncogene products. In lymphocytes and fibroblasts these proteins are different, suggesting that the cascade of events triggered by the v-abl gene in different cell types involves tyrosine phosphorylation of different specific proteins.
- Subjects :
- Phosphopeptides
Cancer Research
Oncogene Proteins
Abelson murine leukemia virus
Biology
SH2 domain
chemistry.chemical_compound
Mice
Protein phosphorylation
hemic and lymphatic diseases
HSPA2
Animals
Chemical Precipitation
Lymphocytes
Tyrosine
Phosphorylation
Phosphotyrosine
Lymphocytes, Fibroblasts, Protein phosphorylation, Abelson murine leukemia virus
Mice, Inbred BALB C
ABL
Proteins
Tyrosine phosphorylation
Oncogene Proteins, Viral
Oncogenes
Protein-Tyrosine Kinases
Fibroblasts
Molecular biology
Molecular Weight
Cell Transformation, Neoplastic
Oncology
Biochemistry
chemistry
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....507685725826de69df112dcf48618be0