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Combination of 3D Printing and ALD for Dentin Fabrication from Dental Pulp Stem Cell Culture
- Source :
- ACS applied bio materials. 4(10)
- Publication Year :
- 2022
-
Abstract
- A combination of fused deposition modeling printing with atomic layer deposition (ALD) of titania was designed to achieve templated biomineralization and terminal odontogenic differentiation of dental pulp stem cells on three-dimensional (3D) printed polylactic acid (PLA) scaffolds. In the absence of the ALD-deposited titania coating, we had previously shown that both plating efficiency and differentiation are adversely impacted when scaffolds are produced by 3D printing rather than traditional polymer molding. These differences were removed when both printed and molded structures were coated with ALD of titania, which improved the outcomes regardless of the manufacturing method. In this case, on all titania-coated substrates, the plating efficiency increased, copious mineral deposition was observed, and RT-PCR indicated a significant upregulation of osteocalcin, a gene associated with mineral deposition. The influence of additional coatings of collagen, gelatin, or fibronectin on the ALD titania-coated and uncoated PLA-printed and molded scaffolds was also investigated. Upregulation of the odontogenic late-stage differentiation sibling protein, dentin sialoprotein, was observed on the collagen ALD-titania-coated scaffolds and to a lesser extent on the gelatin ALD-titania-coated scaffolds.
- Subjects :
- Fabrication
Materials science
Polyesters
Biomedical Engineering
Cell Culture Techniques
3D printing
02 engineering and technology
law.invention
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
Atomic layer deposition
Tissue engineering
Polylactic acid
law
Dentin
medicine
Dental Pulp
030304 developmental biology
0303 health sciences
Fused deposition modeling
Tissue Scaffolds
business.industry
Biochemistry (medical)
General Chemistry
021001 nanoscience & nanotechnology
medicine.anatomical_structure
chemistry
Chemical engineering
Printing, Three-Dimensional
Gelatin
Collagen
0210 nano-technology
business
Biomineralization
Subjects
Details
- ISSN :
- 25766422
- Volume :
- 4
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- ACS applied bio materials
- Accession number :
- edsair.doi.dedup.....507c9204bdeec6fa415195a1c0f4462f