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Over-expression of an electron transport protein OmcS provides sufficient NADH for d-lactate production in cyanobacterium
- Source :
- Biotechnology for Biofuels, Vol 14, Iss 1, Pp 1-16 (2021), Biotechnology for Biofuels
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equivalent. However, most dehydrogenases prefer to utilize NADH as a cofactor. Thus, sufficient NADH supply is crucial to produce dehydrogenase-derived chemicals in cyanobacteria. Photosynthetic electron is the sole energy source and excess electrons are wasted in the light reactions of photosynthesis. Results Here we propose a novel strategy to direct the electrons to generate more ATP from light reactions to provide sufficient NADH for lactate production. To this end, we introduced an electron transport protein-encoding gene omcS into cyanobacterium Synechococcus elongatus UTEX 2973 and demonstrated that the introduced OmcS directs excess electrons from plastoquinone (PQ) to photosystem I (PSI) to stimulate cyclic electron transfer (CET). As a result, an approximately 30% increased intracellular ATP, 60% increased intracellular NADH concentrations and up to 60% increased biomass production with fourfold increased d-lactate production were achieved. Comparative transcriptome analysis showed upregulation of proteins involved in linear electron transfer (LET), CET, and downregulation of proteins involved in respiratory electron transfer (RET), giving hints to understand the increased levels of ATP and NADH. Conclusions This strategy provides a novel orthologous way to improve photosynthesis via enhancing CET and supply sufficient NADH for the photosynthetic production of chemicals.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Plastoquinone
Management, Monitoring, Policy and Law
Photosystem I
Photosynthesis
Cyanobacteria
01 natural sciences
Applied Microbiology and Biotechnology
Cofactor
Electron transfer
03 medical and health sciences
chemistry.chemical_compound
TP315-360
Electron transport protein
Lactate production
biology
Renewable Energy, Sustainability and the Environment
Research
Reducing equivalent
Fuel
Electron transport chain
NADH availability
030104 developmental biology
General Energy
Photosynthetic electron
chemistry
biology.protein
Biophysics
Energy source
TP248.13-248.65
010606 plant biology & botany
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 17546834
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biotechnology for Biofuels
- Accession number :
- edsair.doi.dedup.....50ada2ecc9a9edaad7590b413730b88c