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Effect of prolonged treatment with tyramine on glucose tolerance in streptozotocin-induced diabetic rats
- Source :
- Journal of Physiology and Biochemistry, Journal of Physiology and Biochemistry, Springer Verlag (Germany), 2003, 59 (3), pp.225-232. ⟨10.1007/BF03179919⟩, Journal of Physiology and Biochemistry, 2003, 59 (3), pp.225-232. ⟨10.1007/BF03179919⟩
- Publication Year :
- 2003
- Publisher :
- Springer Verlag, 2003.
-
Abstract
- International audience; The biogenic amine tyramine has been reported to stimulatein vitro glucose transport in adipocytes, cardiomyocytes and skeletal muscle, and to improvein vivo glucose utilization in rats. These effects were dependent on amine oxidation, since they were blocked by inhibitors of monoamine oxidase (MAO) and semicarbazide-sensitive amine oxidase (SSAO). We thus tested in this work whether a prolonged treatment with tyramine could improve glucose tolerance in streptozotocin-induced diabetic rats. First, tyramine content of standard rodent chow was determined by HPLC and daily tyramine intake of control rats was estimated to be around 26 μmol/kg body weight. Then, tyramine was administred during 3 weeks in streptozotocin-induced diabetic rats at 29 μmol/kg by daily i.p. injection alone or together with vanadate 0.02 μmol/kg. In another group of diabetic rats, tyramine was subcutaneously delivered at 116 μmol/kg/day by osmotic minipumps. All tyramine treatments resulted in a decrease of the hyperglycemic responses to an i.p. glucose load. Adipocytes isolated from either untreated or treated diabetic rats were sensitive to the stimulation of glucose uptake by tyramine. However, diabetic animals receiving tyramine for three weeks did not recover from their hyperglycemia, hypoinsulinemia and glucosuria. These results show that the improvement of glucose tolerance induced by prolonged tyramine administration occurs in an insulin-depleted model and probably results from peripheral insulin-like actions of the oxidation of MAO/SSAO substrates, such as the stimulation of glucose uptake into adipocytes.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Semicarbazide-sensitive amine oxidase
Physiology
Monoamine oxidase
Glucose uptake
medicine.medical_treatment
Tyramine
Biochemistry
Diabetes Mellitus, Experimental
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
[SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication
Internal medicine
Diabetes mellitus
Biogenic amine
Adipocytes
medicine
Animals
Génie chimique
Insulin
Rats, Wistar
Génie des procédés
Infusion Pumps
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Chemistry
Diabetes
Glucose transporter
General Medicine
Glucose Tolerance Test
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
medicine.disease
Streptozotocin
3. Good health
Rats
Glucose
Endocrinology
030220 oncology & carcinogenesis
Vanadates
Injections, Intraperitoneal
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 11387548
- Database :
- OpenAIRE
- Journal :
- Journal of Physiology and Biochemistry, Journal of Physiology and Biochemistry, Springer Verlag (Germany), 2003, 59 (3), pp.225-232. ⟨10.1007/BF03179919⟩, Journal of Physiology and Biochemistry, 2003, 59 (3), pp.225-232. ⟨10.1007/BF03179919⟩
- Accession number :
- edsair.doi.dedup.....50f34f93f34500b11c195e0c601a2cff