Additional file 1 of Long non-coding RNA H19 regulates matrisome signature and impacts cell behavior on MSC-engineered extracellular matrices

Additional file 1: Fig. S1. Effect of siH19-2 on the expression levels of collagens, osteogenic markers and other H19-target candidates, assessed through RT-qPCR, 3 days after culture under osteogenic-inducing conditions (N = 6). Fig. S2. Decellularization of MSCs matrices. Primary MSCs from osteoporotic patients were differentiated for 10 days under osteogenic-inducing conditions before decellularization. After treatment with the decellularization buffer (urea 2 M), samples were stained with a) DAPI (blue) to visualize nuclei (scale: 100 μm) and b) hematoxylin (blue-purple color) to visualize nuclei and matrix integrity (scale: 100 μm). c) DNA and sGAGs content were measured. Non-decellularized matrices were used as a control. Fig. S3. In silico predictions for the binding site between miR-29-a/b/c and lncRNA H19. Fig. S4. Expression profile of H19 during early osteogenic differentiation stages in MSCs from healthy donors (N = 4) and osteoporotic patients (N = 4). Fig. S5. miR-29c-3p expression after transfection of MSCs with miR-29c-3p mimics, miR-29c-3p inhibitor or respective controls (NC-mimics and NC-inhibitor), and cultured for 3 days in osteogenic-inducing conditions (N = 6). Table SI. Donors of Human Mesenchymal Stem/Stromal Cells. Table SII. Primers used for reverse transcription quantitative real-time PCR. Table SIII. Mature miRNAs sequences according to miRbase annotations ( http://www.mirbase.org/ ). Table SIV. Antibodies used for immunocytochemistry stainings.