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Genomic sequence analysis of granulovirus isolated from the tobacco cutworm, Spodoptera litura
- Source :
- PLoS ONE, PLoS ONE, Vol 6, Iss 11, p e28163 (2011)
- Publication Year :
- 2011
-
Abstract
- BACKGROUND: Spodoptera litura is a noctuid moth that is considered an agricultural pest. The larvae feed on a wide range of plants and have been recorded on plants from 40 plant families (mostly dicotyledons). It is a major pest of many crops. To better understand Spodoptera litura granulovirus (SpliGV), the nucleotide sequence of the SpliGV DNA genome was determined and analyzed. METHODOLOGY/PRINCIPAL FINDINGS: The genome of the SpliGV was completely sequenced. The nucleotide sequence of the SpliGV genome was 124,121 bp long with 61.2% A+T content and contained 133 putative open reading frames (ORFs) of 150 or more nucleotides. The 133 putative ORFs covered 86.3% of the genome. Among these, 31 ORFs were conserved in most completely sequenced baculovirus genomes, 38 were granulovirus (GV)-specific, and 64 were present in some nucleopolyhedroviruses (NPVs) and/or GVs. We proved that 9 of the ORFs were SpliGV specific. CONCLUSIONS/SIGNIFICANCE: The genome of SpliGV is 124,121 bp in size. One hundred thirty-three ORFs that putatively encode proteins of 50 or more amino acid residues with minimal overlap were determined. No chitinase or cathepsin genes, which are involved in the liquefaction of the infected host, were found in the SpliGV genome, explaining why SpliGV-infected insects do not degrade in a typical manner. The DNA photolyase gene was first found in the genus Granulovirus. When phylogenic relationships were analyzed, the SpliGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XecnGV), which belong to the Type I-granuloviruses (Type I-GV).
- Subjects :
- Baculoviridae
Genes, Viral
Transcription, Genetic
Agricultural Biotechnology
lcsh:Medicine
Spodoptera litura
Plant Science
Inhibitor of Apoptosis Proteins
Gene Order
Genome Sequencing
lcsh:Science
Phylogeny
Genetics
Plant Pests
Multidisciplinary
biology
Reverse Transcriptase Polymerase Chain Reaction
Nucleic acid sequence
Agriculture
Genomics
Research Article
DNA Replication
Gene Expression Regulation, Viral
Sequence analysis
Molecular Sequence Data
Granulovirus
Genome, Viral
Spodoptera
Microbiology
Cutworm
Open Reading Frames
Sequence Homology, Nucleic Acid
Virology
Botany
Tobacco
Animals
RNA, Messenger
Biology
Base Sequence
lcsh:R
fungi
Computational Biology
Sequence Analysis, DNA
Plant Pathology
biology.organism_classification
lcsh:Q
PEST analysis
Pest Control
Zoology
Entomology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....50fee448a74efb4ff36eb03d6d0b91f2