Back to Search
Start Over
Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise
- Source :
- The Journal of Physiological Sciences
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- The aim of this study was to clarify the intracellular β2-adrenergic receptor signaling specificity in mouse slow-twitch soleus and fast-twitch tibialis anterior (TA) muscles, resulting from single-dose β2-agonist clenbuterol treatment and acute exercise. At 1, 4, and 24 h after single-dose treatment with clenbuterol or after acute running exercise, the soleus and TA muscles were isolated and subjected to analysis. The phosphorylation of p38 mitogen-activated protein kinase (MAPK) increased after single-dose clenbuterol treatment and acute exercise in the soleus muscle but not in the TA muscle. Although there was no change in the phosphorylation of Akt after acute exercise in either muscle, phosphorylation of Akt in the soleus muscle increased after single-dose clenbuterol treatment, whereas that in the TA muscle remained unchanged. These results suggest that p38 MAPK and Akt pathways play a functional role in the adaptation to clenbuterol treatment and exercise, particularly in slow-twitch muscles.
- Subjects :
- Male
MAPK/ERK pathway
β2-Agonist clenbuterol
medicine.medical_specialty
Physiology
Short Communication
p38 mitogen-activated protein kinases
Skeletal muscle
p38 MAPK
p38 Mitogen-Activated Protein Kinases
Mice
β2-Adrenergic receptor signaling
Physical Conditioning, Animal
Internal medicine
medicine
Animals
Clenbuterol
Phosphorylation
Muscle, Skeletal
Protein kinase A
Exercise
Adrenergic beta-2 Receptor Agonists
Protein kinase B
Soleus muscle
business.industry
Akt
musculoskeletal system
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Mice, Inbred C57BL
Muscle Fibers, Slow-Twitch
Endocrinology
medicine.anatomical_structure
Muscle Fibers, Fast-Twitch
business
Proto-Oncogene Proteins c-akt
Transcription Factors
medicine.drug
Subjects
Details
- ISSN :
- 18806562 and 18806546
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- The Journal of Physiological Sciences
- Accession number :
- edsair.doi.dedup.....51005a8ede31b8df1a3e898a2f7547bc