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Authentication and Assessment of Contamination in Ancient DNA

Authors :: Gabriel Renaud
Mikkel Schubert
Susanna Sawyer
Ludovic Orlando
Max Planck Institute for Evolutionary Anthropology [Leipzig]
Max-Planck-Gesellschaft
University of Copenhagen = Københavns Universitet (KU)
Section for GeoGenetics
Globe Institute
Faculty of Health and Medical Sciences
University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)-Faculty of Health and Medical Sciences
University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)
Shapiro, Beth
Barlow, Axel
Heintzman, Peter D.
Hofreiter, Michael
Paijmans, Johanna L. A.
Soares, André E. R.
Source :: Ancient DNA, Ancient DNA, pp.163-194, 2019, ⟨10.1007/978-1-4939-9176-1_17⟩, Methods in Molecular Biology, Methods in Molecular Biology-Ancient DNA, Ancient DNA-Methods and Protocols, Renaud, G, Schubert, M, Sawyer, S & Orlando, L 2019, Authentication and assessment of contamination in ancient DNA . in B Shapiro, A Barlow, P D Heintzman, M Hofreiter, J L A Paijmans & A E R Soares (eds), Ancient DNA : Methods and Protocols . 2. ed. edn, Humana Press, Methods in Molecular Biology, vol. 1963, pp. 163-194 . https://doi.org/10.1007/978-1-4939-9176-1_17, Methods in Molecular Biology ISBN: 9781493991754
Publication Year :: 2019
Abstract: Contamination from both present-day humans and postmortem microbial sources is a common challenge in ancient DNA studies. Here we present a suite of tools to assist in the assessment of contamination in ancient DNA data sets. These tools perform standard tests of authenticity of ancient DNA data including detecting the presence of postmortem damage signatures in sequence alignments and quantifying the amount of present-day human contamination.