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Complete amino acid sequence of an integrin beta subunit (beta 7) identified in leukocytes
- Source :
- Europe PubMed Central
- Publication Year :
- 1991
-
Abstract
- The integrins are a large family of heterodimeric cell-surface glycoproteins that play key roles in the adherence of cells to other cells and to extracellular matrix proteins. We have previously reported the identification of a novel integrin beta subunit partial cDNA from leukocytes. We have now determined the complete sequence of this subunit, designated as beta 7, from overlapping clones obtained from a PEER T leukemia cell library and a peripheral T cell library. The beta 7 cDNA contains a single large open reading frame predicted to encode a 798-amino acid protein precursor (signal peptide plus mature protein). The beta 7 protein, like the other beta subunit proteins, is predicted to contain a large extracellular portion, a transmembrane domain, and a cytoplasmic tail. The deduced beta 7 amino acid sequence is 32-46% identical to the six previously sequenced human integrin beta subunits. beta 7 is most similar to the leukocyte integrin common beta subunit (beta 2, CD18). Analysis of variant beta 7 cDNA clones and reverse transcription-polymerase chain reaction products suggest that alternatively spliced beta 7 mRNAs can be generated by the removal of exons that encode most of the cysteine-rich region of the extracellular portion of beta 7. By Northern blot analysis, beta 7 mRNA was detected in T and B cell lines and in macrophage-like cell lines, but not in any of the nonleukocyte cell lines tested. Peripheral T cells and some lymphoma lines express little beta 7 mRNA before stimulation; but after stimulation with phorbol ester, beta 7 mRNA levels increased markedly. Integrin beta 7 is expected to play a role in adhesive interactions of leukocytes.
- Subjects :
- Integrins
Leukemia, T-Cell
Macromolecular Substances
Protein subunit
T-Lymphocytes
Molecular Sequence Data
Restriction Mapping
Biology
Biochemistry
Polymerase Chain Reaction
Cell Line
Sequence Homology, Nucleic Acid
Humans
Amino Acid Sequence
Cloning, Molecular
Beta (finance)
Molecular Biology
Peptide sequence
Interleukin 12 receptor, beta 1 subunit
Gene Library
Base Sequence
Cell Biology
DNA, Neoplasm
TGF beta receptor 2
Molecular biology
Transmembrane domain
biology.protein
Integrin, beta 6
DNA Probes
Oligonucleotide Probes
ATP synthase alpha/beta subunits
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 266
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....51ac62c0827dacc0bff00ca4c1542cd4