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Genome-wide identification and comprehensive analyses of NAC transcription factor gene family and expression patterns during somatic embryogenesis in Dimocarpus longan Lour
- Source :
- Plant Physiology and Biochemistry. 157:169-184
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The NAM, ATAF1/2, and CUC2 form a huge plant-specific gene family of NAC TFs that are involved in the growth, development, and regulation of biotic and abiotic stress responses. Although the draft genome of longan (Dimocarpus longan Lour.) has been published, however the comprehensive data regarding the functions, evolution, and expression patterns of the NAC family are still unavailable. In this study, a comprehensive analysis of the NAC transcription factor family in longan was performed, and a total of 114 NAC genes were found. We investigated the NAC gene family exploring the phylogeny, domain conservation, intron/exon, motifs, cis-regulatory elements, protein-protein interaction, and expression profiles of RNA-seq samples in different tissues and early somatic embryogenesis of longan. Phylogenetic analysis showed that the genes with similar gene structure and motif distribution were clustered in the same group. Cis-element identification indicates the possible role of NAC genes in biological and physiological processes. Protein-protein interaction identified the DlNACs homologous with Arabidopsis proteins. We further investigated the expression pattern of DlNAC genes in different tissues (pulp, stem, large fruit, young fruit, and flower) during somatic embryogenesis at embryogenic callus (EC), incomplete compact pro-embryogenic cultures (ICpEC), and globular embryos (GE) stages. The qRT-PCR results showed that the DlNAC genes were expressed higher at EC and GE stage compared with ICpEC stage. In conclusion, our results provide insight into the evolution, diversity, and characterization of NAC genes in the longan and provide a base for understanding their biological roles and molecular mechanisms in plants.
- Subjects :
- Plant Somatic Embryogenesis Techniques
0106 biological sciences
0301 basic medicine
Somatic embryogenesis
Physiology
Plant Science
Biology
01 natural sciences
Genome
03 medical and health sciences
Sapindaceae
Gene Expression Regulation, Plant
Gene expression
Genetics
Gene family
Transcription factor
Gene
Phylogeny
Plant Proteins
Gene Expression Profiling
Intron
030104 developmental biology
Multigene Family
Transcription Factor Gene
Transcription Factors
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09819428
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- Plant Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....51b568e0a9b8f4c6cf3f09eab2eb413b