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Platinum particles-modified electrode for HPLC with pulsed amperometric detection of thiols in rat striatum
- Source :
- Biomedical chromatography : BMC. 18(8)
- Publication Year :
- 2004
-
Abstract
- A new chemically modified electrode based on the immobilization of Pt particles is fabricated and exhibits electrocatalytic oxidation for l-cysteine (l-Cys), glutathione (GSH) and penicillamine (PEN) with relatively high sensitivity. It is also adaptable to HPLC for pulsed amperometric detection (PAD) of these thiols. PAD largely improves the detection sensitivity because the alternated polarizations can effectively clean and reactivate the electrode surface. It is shown that the peak currents of l-Cys, GSH and PEN are linear to their concentrations, with the calculated detection limit of 1.1 × 10−7, 1.8 × 10−7 and 3.8 × 10−7 mol L−1, respectively (S/N = 3). The method has been successfully applied to assess the contents of l-Cys and GSH in rat striatal microdialysates. The average contents of the two analytes in rat striatum are 2.6 × 10−6 and 2.8 × 10−6 mol L−1, respectively. Copyright © 2004 John Wiley & Sons, Ltd.
- Subjects :
- Analyte
Time Factors
Clinical Biochemistry
chemistry.chemical_element
Biochemistry
High-performance liquid chromatography
Sensitivity and Specificity
Analytical Chemistry
Rat striatum
Rats, Sprague-Dawley
chemistry.chemical_compound
Drug Discovery
Electrochemistry
Animals
Cysteine
Sulfhydryl Compounds
Molecular Biology
Electrodes
Chromatography, High Pressure Liquid
Platinum
Pharmacology
Detection limit
Chromatography
Penicillamine
Reproducibility of Results
General Medicine
Glutathione
Amperometry
Corpus Striatum
Rats
chemistry
Electrode
Oxidation-Reduction
Subjects
Details
- ISSN :
- 02693879
- Volume :
- 18
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biomedical chromatography : BMC
- Accession number :
- edsair.doi.dedup.....51b915f6d6e4e2753c130943cdbd3ce8