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Silver nanoparticles synthesized from Adenium obesum leaf extract induced DNA damage, apoptosis and autophagy via generation of reactive oxygen species
- Source :
- Colloids and surfaces. B, Biointerfaces. 141
- Publication Year :
- 2015
-
Abstract
- Silver nanoparticles (AgNPs) are an important class of nanomaterial used for a wide range of industrial and biomedical applications. Adenium obesum is a plant of the family Apocynaceae that is rich in toxic cardiac glycosides; however, there is scarce information on the anticancer potential of its AgNPs. We herein report the novel biosynthesis of AgNPs using aqueous leaf extract of A. obesum (AOAgNPs). The synthesis of AOAgNPs was monitored by color change and ultraviolet-visible spectroscopy (425 nm). It was further characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). The FTIR spectra for the AOAgNPs indicated the presence of terpenoids, long chain fatty acids, secondary amide derivatives and proteins that could be responsible for the reduction and capping of the formed AOAgNPs. X-ray diffraction confirmed the crystallinity of the AgNPs. The TEM images revealed mostly spherical particles in the size range of 10-30 nm. The biological properties of novel AOAgNPs were investigated on MCF-7 breast cancer cells. Cell viability was determined by the MTT assay. Generation of reactive oxygen species (ROS), DNA damage, induction of apoptosis and autophagy were assessed. A dose-dependent decrease in the cell viability was observed. The IC50 value was calculated as 217 μg/ml. Both qualitative and quantitative evaluation confirmed about a 2.5 fold increase in the generation of ROS at the highest concentration of 150 μg/ml. A significant (p
- Subjects :
- 0301 basic medicine
Silver
DNA damage
Cell Survival
Metal Nanoparticles
Apoptosis
Breast Neoplasms
02 engineering and technology
Biology
Silver nanoparticle
03 medical and health sciences
chemistry.chemical_compound
Colloid and Surface Chemistry
Microscopy, Electron, Transmission
X-Ray Diffraction
Adenium obesum
Spectroscopy, Fourier Transform Infrared
Autophagy
Humans
MTT assay
Viability assay
Physical and Theoretical Chemistry
chemistry.chemical_classification
Reactive oxygen species
Dose-Response Relationship, Drug
Plant Extracts
Acridine orange
Surfaces and Interfaces
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Comet assay
Apocynaceae
Plant Leaves
030104 developmental biology
Biochemistry
chemistry
Microscopy, Fluorescence
MCF-7 Cells
Comet Assay
0210 nano-technology
Reactive Oxygen Species
Biotechnology
DNA Damage
Subjects
Details
- ISSN :
- 18734367
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Accession number :
- edsair.doi.dedup.....51c5d3eafdb4990c51ab29279d06f927