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Integrated bioinformatics analysis revealed the regulation of angiogenesis by tumor cells in hepatocellular carcinoma
- Source :
- Bioscience Reports
- Publication Year :
- 2021
- Publisher :
- Portland Press Ltd., 2021.
-
Abstract
- Hepatocellular carcinoma (HCC) is one of the leading causes of cancer mortality, metastasis accounts for most of the cases. Angiogenesis plays an important role in cancer metastasis, but how tumor cells affect the function of endothelial cells by dictating their microRNA (miRNA) expression remains largely unknown. Differentially expressed miRNAs (DEMs) were identified through dataset downloaded from the Gene Expression Omnibus (GEO) database and analyzed by GEO2R. We then used online tools to obtain potential targets of candidate miRNAs and functional enrichment analysis, as well as the protein-protein interaction (PPI). Finally, the function of miR-302c-3p was validated through in vitro assay. In the current study, we found that HCC cells altered miRNA expression profiles of human umbilical vein endothelial cells (HUVECs) and miR-302c-3p was the most down-regulated miRNA in HUVECs when they were co-cultured with HCC-LM3 cells. Functional enrichment analysis of the candidate targets revealed that these genes were involved in epigenetic regulation of gene expression, in particular, cytosine methylation. In addition, PPI network demonstrated distinct roles of genes targeted by miR-302c-3p. Importantly, inhibition of angiogenesis, migration and permeability by the most down-regulated miR-302c-3p in HUVECs was confirmed in vitro. These findings brought us novel insight into the regulation of angiogenesis by HCC cells and provided potential targets for the development of therapeutic strategies.
- Subjects :
- 0301 basic medicine
Carcinoma, Hepatocellular
Angiogenesis
Bioinformatics
Biophysics
Biology
Biochemistry
Permeability
Metastasis
Epigenesis, Genetic
03 medical and health sciences
angiogenesis
0302 clinical medicine
Cell Movement
Cell Line, Tumor
microRNA
Gene expression
Databases, Genetic
medicine
Biomarkers, Tumor
Humans
Epigenetics
Protein Interaction Maps
Molecular Biology
Gene
Research Articles
Cancer
Neovascularization, Pathologic
Liver Neoplasms
Endothelial Cells
Cell Biology
hepatocellular carcinoma
medicine.disease
GEO
In vitro
bioinformatic analysis
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
DNA methylation
Cancer research
Cell Migration, Adhesion & Morphology
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 41
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....51cee663f2d4c3e846eaa0da56d8b74d