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Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro
- Source :
- Oncotarget
- Publication Year :
- 2016
- Publisher :
- Impact Journals, LLC, 2016.
-
Abstract
- Excretory and secretory products (ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products (HcESPs) were collected from the adult worms. Binding of HcESPs to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immune-fluorescence assay. Effects of the HcESPs on cytokine production, cell proliferation, cell migration and nitric oxide (NO) production of PBMCs were checked by co-incubation of HcESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by HcESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by HcESPs, whereas, HcESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the HcESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.
- Subjects :
- Male
0301 basic medicine
medicine.medical_treatment
Fluorescent Antibody Technique
Biology
Nitric Oxide
immunomodulation
Peripheral blood mononuclear cell
Host-Parasite Interactions
Microbiology
Interferon-gamma
03 medical and health sciences
Immune system
Cell Movement
Haemonchus contortus
medicine
Animals
Immune response
ESP
Cells, Cultured
Interleukin 4
Cell Proliferation
Cell growth
Goats
Interleukin-17
goat
PBMC
Research Paper: Immunology
Immunity
Helminth Proteins
biology.organism_classification
Interleukin-10
Interleukin 10
030104 developmental biology
Secretory protein
Cytokine
Oncology
Immunology
Leukocytes, Mononuclear
Immunology and Microbiology Section
Haemonchus
Interleukin-4
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....51d845c83055d817f2da66ff53fc9988