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Structural and functional analysis of the surface protein of human coronavirus OC43
- Source :
- Virology
- Publication Year :
- 1993
-
Abstract
- The two surface glycoproteins S and HE of human coronavirus OC43 (HCV-OC43) were isolated from the viral membrane and purified. Only the S protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of HCV-OC43. The receptor determinant recognized by this virus on the surface of erythrocytes is N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) which is also used by bovine coronavirus for attachment to cells. By analyzing erythrocytes containing different amounts of Neu5,9Ac2 in either of two linkage types, it was found that there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid. Bovine coronavirus was more efficient in recognizing low amounts of Neu5,9Ac2 alpha 2,3 linked to galactose, whereas HCV-OC43 was superior with respect to the alpha 2,6 linkage. The gene coding for the S protein of HCV-OC43 was cloned and sequenced. A large open reading frame predicts a polypeptide of 150 kDa in the unglycosylated form. A protein of about 190 kDa is expected if the 20 potential glycosylation sites are used for attachment of N-linked oligosaccharide side chains. These predictions were confirmed by in vitro transcription and translation of the gene in the presence or absence of canine pancreatic microsomal membranes. A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus. Structural and functional analyses of more strains should help to identify the location of the sialic acid-binding site.
- Subjects :
- Viral protein
Coronaviridae
viruses
Molecular Sequence Data
Hemagglutinin (influenza)
Hemagglutinins, Viral
medicine.disease_cause
Article
chemistry.chemical_compound
Viral Envelope Proteins
Virology
Agglutination Tests
Viral structural protein
medicine
Tumor Cells, Cultured
Animals
Humans
Human coronavirus OC43
Amino Acid Sequence
Cloning, Molecular
Bovine coronavirus
Coronavirus
Membrane Glycoproteins
biology
Base Sequence
virus diseases
biology.organism_classification
N-Acetylneuraminic Acid
Sialic acid
chemistry
Biochemistry
DNA, Viral
Spike Glycoprotein, Coronavirus
biology.protein
Sialic Acids
N-Acetylneuraminic acid
Chickens
Protein Binding
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 195
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....51d899202636c0e4abccc5a6427c4ec5