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Structure of the Porcine Vasoactive Intestinal Octacosapeptide. The Amino-Acid Sequence. Use of Kallikrein in Its Determination
- Source :
- European Journal of Biochemistry. 42:581-589
- Publication Year :
- 1974
- Publisher :
- Wiley, 1974.
-
Abstract
- The amino acid sequence of the porcine vasoactive intestinal octacosapeptide is His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-L ys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2. Its amino acid residues 1, 2, 6 and 7, counted from the N-terminus, are identical to those in the corresponding positions in both porcine glucagon and secretin. The residues in positions 3, 12, 13, 14 and 23 are identical to those in secretin, but not in glucagon, and position 10 is occupied by a tyrosyl and position 28 by an asparaginyl residue, like in glucagon but not in secretin. If in addition to identical amino acid residues also chemically similar residues, such as isoleucine in position 26 of the octacosapeptide, as compared to leucine in secretin and glucagon, are taken into consideration then the similarity between these three polypeptides is still more evident. At a more remote level there is some structural resemblance also between these peptides and the other four peptides from the intestinal wall, cholecystokinin-pancreozymin, motilin, the gastric inhibitory peptide and substance P, the structures of which are known. The elucidation of the structure of the octacosapeptide was facilitated by the finding that pancreatic kallikrein preferentially cleaved only one of the three bonds in its N-terminal cyanogen bromide heptadecapeptide that are susceptible to cleavage with trypsin.
- Subjects :
- Chromatography, Paper
Swine
Vasopressins
Aminopeptidases
Biochemistry
Glucagon
Chromatography, DEAE-Cellulose
Secretin
Gastrointestinal Hormones
chemistry.chemical_compound
medicine
Animals
Electrophoresis, Paper
Amino Acid Sequence
Cyanogen Bromide
Amino Acids
Peptide sequence
Dansyl Compounds
Kallikrein
Chromatography, Ion Exchange
Trypsin
Peptide Fragments
Intestines
chemistry
Kallikreins
Spectrophotometry, Ultraviolet
Cyanogen bromide
Leucine
Isoleucine
Peptides
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Subjects
Details
- ISSN :
- 14321033 and 00142956
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- European Journal of Biochemistry
- Accession number :
- edsair.doi.dedup.....51db160f31a0fff349a2ef1926eed4a7