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Versatile High Resolution Oligosaccharide Microarrays for Plant Glycobiology and Cell Wall Research
- Source :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (47), ⟨10.1074/jbc.M112.396598⟩, Journal of Biological Chemistry 47 (287), . (2012)
- Publication Year :
- 2012
- Publisher :
- HAL CCSD, 2012.
-
Abstract
- International audience; Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts of analytes. Nucleotide microarrays are well established in plant research, but carbohydrate microarrays are much less established, and one reason for this is a lack of suitable glycans with which to populate arrays. Polysaccharide microarrays are relatively easy to produce because of the ease of immobilizing large polymers noncovalently onto a variety of microarray surfaces, but they lack analytical resolution because polysaccharides often contain multiple distinct carbohydrate substructures. Microarrays of defined oligosaccharides potentially overcome this problem but are harder to produce because oligosaccharides usually require coupling prior to immobilization. We have assembled a library of well characterized plant oligosaccharides produced either by partial hydrolysis from polysaccharides or by de novo chemical synthesis. Once coupled to protein, these neoglycoconjugates are versatile reagents that can be printed as microarrays onto a variety of slide types and membranes. We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes.
- Subjects :
- 0106 biological sciences
EXPRESSION
Glycan
Microarray
MONOCLONAL-ANTIBODY
DIVERSITY
Glycobiology and Extracellular Matrices
PROTEIN
METABOLISM
Polysaccharide
01 natural sciences
Biochemistry
Glycomics
POLYSACCHARIDES
03 medical and health sciences
PECTIN
Cell Wall
[SDV.IDA]Life Sciences [q-bio]/Food engineering
LOCATION
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Molecular Biology
SPECIFICITY
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Glycobiology
CARBOHYDRATE-BINDING MODULES
Cell Biology
Plants
Oligosaccharide
Microarray Analysis
chemistry
Protein microarray
biology.protein
DNA microarray
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (47), ⟨10.1074/jbc.M112.396598⟩, Journal of Biological Chemistry 47 (287), . (2012)
- Accession number :
- edsair.doi.dedup.....520605d28cdfa64e337b9846e194e871
- Full Text :
- https://doi.org/10.1074/jbc.M112.396598⟩