Additional file 2 of Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

Additional file 2: Supplementary Figure 2. (A) Triple-coated or anti-PE beads were incubated for 10, 25 or 60 min in plasma (donor 6) spiked with HT29-CFSE EVs. Fluorescence signals were read on beads and specificity (S) determined. Highest average S/N ratios were obtained at 60min. Experiment performed in duplicate. (B) HT29-CFSE EVs were spiked in plasma (donor 6) diluted 1:2, 1:5, 1:10 or 1:20 with PBS, whilst maintaining a constant volume for IP with triple-coated beads. S/N ratios indicated that a similar recovery was achieved across dilutions. Experiment performed in duplicate.