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Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report
- Source :
- American Journal of Respiratory Cell and Molecular Biology
- Publication Year :
- 2019
- Publisher :
- American Thoracic Society, 2019.
-
Abstract
- Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Lung Diseases
medicine.medical_specialty
media_common.quotation_subject
Clinical Biochemistry
Apoptosis
Cell Separation
lung biology
Flow cytometry
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
Quality (business)
Myeloid Cells
Intensive care medicine
Molecular Biology
Pulmonary flow
reproducibility
Lung
Organ system
Societies, Medical
media_common
American Thoracic Society Documents
Reproducibility
medicine.diagnostic_test
business.industry
flow cytometry
Reproducibility of Results
Cell Biology
Limiting
Congresses as Topic
United States
030104 developmental biology
medicine.anatomical_structure
Phenotype
030228 respiratory system
Practice Guidelines as Topic
cells
business
Cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 15354989 and 10441549
- Volume :
- 61
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- American Journal of Respiratory Cell and Molecular Biology
- Accession number :
- edsair.doi.dedup.....52320b34bddc75864ad9e4d194a46e84