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Apocynin Ameliorates Testicular Toxicity in High-Fat Diet-Fed Rats By Regulating Oxidative Stress
- Source :
- Clinical and Experimental Health Sciences. 13:75-83
- Publication Year :
- 2023
- Publisher :
- Marmara University, 2023.
-
Abstract
- Objective: The purpose of this study was to examine the effects of apocynin (APC), an inhibitor of NADPH oxidase (NOX), on high-fat diet (HF)-induced testis cytotoxicity. Methods: Wistar albino rats were divided into three groups as control, HF and HF+APC groups. Rats in HF and HF+APC groups were fed using HF for 16 weeks and in the last four weeks of this period vehicle solution or APC (25 mg/kg) was administered orally five days a week, respectively. Control group was fed with standart lab chow for 16 weeks. Cholesterol, triglyceride, high-density lipoproteins, leptin, estrogen, testosterone, LH and FSH were estimated in blood serum. Sperm parameters were analysed from the epididymis. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, glutathione, superoxide dismutase and myeloperoxidase levels were estimated biochemically. Testicular morphology, proliferative, apoptotic and NOX2-positive cells were analysed histologically. HF-induced obesity caused significant alterations in serum lipid and hormone profiles. Testicular malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, and myeloperoxidase levels increased, glutathione and superoxide dismutase levels decreased in this group. Moreover, altered sperm parameters, increased degenerated seminiferous tubules, apoptotic and NOX2- positive cells and decreased proliferative cells were observed in the HF group. All these biochemical and histological alterations improved in the HF+APC group. Conclusion: HF-induced obesity causes altreations in lipid values, sperm parameters and testicular morphology by increasing oxidative stress through NOX2 activity. Apocynin might prevent testis damage via regulating oxidant/antioxidant balance.
- Subjects :
- Temel Tıp Bilimleri
Histoloji-Embriyoloji
Life Sciences (LIFE)
Medicine (miscellaneous)
CELL BIOLOGY
Assessment and Diagnosis
testis
Sağlık Bilimleri
Temel Bilgi ve Beceriler
Genel Tıp
Fundamental Medical Sciences
Pathophysiology
Clinical Medicine (MED)
TIP, GENEL & DAHİLİ
apocynin
Health Sciences
Yaşam Bilimleri
Internal Medicine
oxidative stress
Klinik Tıp (MED)
Aile Sağlığı
MEDICINE, GENERAL & INTERNAL
Moleküler Biyoloji ve Genetik
Dahiliye
Patofizyoloji
Klinik Tıp
Hücre Biyolojisi
Histology and Embryology
High fat diet
Fundamentals and Skills
apoptosis
Life Sciences
General Medicine
CLINICAL MEDICINE
HÜCRE BİYOLOJİSİ
Değerlendirme ve Teşhis
Tıp
MOLECULAR BIOLOGY & GENETICS
Yaşam Bilimleri (LIFE)
General Health Professions
Medicine
Tıp (çeşitli)
Family Practice
Genel Sağlık Meslekleri
Subjects
Details
- ISSN :
- 24591459
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Health Sciences
- Accession number :
- edsair.doi.dedup.....52458b36455fb422bb32c61296f810aa