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MMP-7 and fcDNA Serum Levels in Early NSCLC and Idiopathic Interstitial Pneumonia: Preliminary Study
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 14, Iss 12, Pp 24097-24112 (2013), Volume 14, Issue 12, Pages 24097-24112
- Publication Year :
- 2013
- Publisher :
- Molecular Diversity Preservation International (MDPI), 2013.
-
Abstract
- A non-invasive test to facilitate the diagnosis of non-small cell lung cancer (NSCLC) and idiopathic pulmonary fibrosis (IPF) is still not available and represents an important goal. Forty-eight patients with stage I NSCLC, 45 with IPF, 30 with other idiopathic interstitial pneumonias (IIPs) including idiopathic non-specific interstitial pneumonia (NSIP) and chronic hypersensitivity pneumonitis (HP), 35 with diffuse non-malignant disease and 30 healthy donors were enrolled onto the study. Free circulating (fc)DNA and MMP-7 levels were evaluated by Real Time PCR and ELISA, respectively. Median fcDNA levels were similar in NSCLC (127 ng/mL, range 23.6–345 ng/mL) and IPF (106 ng/mL, range 22–224 ng/mL) patients, and significantly lower in IIPs patients, in individuals with other diseases and in healthy donors (p &lt<br />0.05). Conversely, median MMP-7 values were significantly higher in IPF patients (9.10 ng/mL, range 3.88–19.72 ng/mL) than in those with NSCLC (6.31 ng/mL, range 3.38–16.36 ng/mL<br />p &lt<br />0.0001), NSIP (6.50 ng/mL, range 1.50–22.47 ng/mL<br />p = 0.007), other diseases (5.41 ng/mL, range 1.78–15.91, p &lt<br />0.0001) or healthy donors (4.35 ng/mL, range 2.45–7.23<br />0.0001). Serum MMP-7 levels seem to be capable of distinguishing IPF patients from those with any other lung disease. fcDNA levels were similar in NSCLC and IPF patients, confirming its potential role as a biomarker, albeit non-specific, for the differential diagnosis of NSCLC.
- Subjects :
- Male
Pathology
Lung Neoplasms
NSIP
Matrix metalloproteinase
NSCLC
Gastroenterology
lcsh:Chemistry
Idiopathic pulmonary fibrosis
Carcinoma, Non-Small-Cell Lung
lcsh:QH301-705.5
Spectroscopy
General Medicine
respiratory system
Middle Aged
Computer Science Applications
Real-time polymerase chain reaction
Area Under Curve
Matrix Metalloproteinase 7
IIPs
Female
Non small cell
Hypersensitivity pneumonitis
MMP-7
medicine.medical_specialty
fcDNA
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Catalysis
Article
Inorganic Chemistry
Diagnosis, Differential
Internal medicine
medicine
Humans
Interstitial pneumonia
Idiopathic Interstitial Pneumonias
Physical and Theoretical Chemistry
Molecular Biology
Idiopathic interstitial pneumonia
Aged
business.industry
Organic Chemistry
DNA
medicine.disease
Idiopathic Pulmonary Fibrosis
respiratory tract diseases
IPF
lcsh:Biology (General)
lcsh:QD1-999
ROC Curve
business
serum
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 14
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....524f2813658bb561f2f3e269bde185bc