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Insulin and Insulin-like growth factor-1 can activate the phosphoinositide-3-kinase /Akt/FoxO1 pathway in T cells in vitro
- Source :
- Dermato-endocrinology
- Publication Year :
- 2017
- Publisher :
- Informa UK Limited, 2017.
-
Abstract
- Hyper-glycemic food increases insulin-like growth factor 1 (IGF-1) and insulin signaling and regulates endocrine responses and thereby may modulate the course of acne. Inflammation and adaptive immune responses have a pivotal role in all stages of acne. Recent hypothesis suggests that hyperglycemic food reduces nuclear forkhead box-O1 (FoxO1) transcription factor and may eventually induces acne. The aim of our study was to investigate the role of IGF-1 and insulin on the phosphoinositide-3-kinase (PI3K)/Akt/FoxO1 pathway in human primary T cells and on the molecular functions of T cells in vitro. T cells were stimulated with 0.001 μM IGF-1 or 1 μM insulin +/− 20 μM PI3K inhibitor LY294002. T cells were also exposed to SZ95 sebocyte supernatants which were pre-stimulated with IGF-1 or insulin. We found that 0.001 µM IGF-1 and 1 µM insulin activate the PI3K pathway in T cells leading to up-regulation of p-Akt and p-FoxO1 at 15 and 30 minutes. Nuclear FoxO1 was decreased and FoxO transcriptional activity was reduced. 0.001 µM IGF-1 and 1 µM insulin increased T cell proliferation but have no significant effect on Toll-like receptor2/4 (TLR) expression. Interestingly, supernatants from IGF-1- or insulin-stimulated sebocytes activated the PI3K pathway in T cells but reduced T cell proliferation. Taken together, this study helps to support that high glycemic load diet may contribute to induce activation of the PI3K pathway and increase of proliferation in human primary T cells. Factors secreted by IGF-1- and insulin-stimulated sebocytes induce the PI3K pathway in T cells and reduce T cell proliferation, which probably can reflect a protective mechanism of the sebaceous gland basal cells.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
sebocytes
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
T cells
FOXO1
Inflammation
Dermatology
Biology
PI3K
High glycaemic load diet
03 medical and health sciences
Insulin-like growth factor
Internal medicine
medicine
PI3K/AKT/mTOR pathway
Insulin
Growth factor
In vitro
Insulin receptor
030104 developmental biology
Endocrinology
Acne
FoxO1
IGF-1
biology.protein
medicine.symptom
Research Paper
Subjects
Details
- ISSN :
- 19381980
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Dermato-Endocrinology
- Accession number :
- edsair.doi.dedup.....527ab56c8b901b32dc904ef9a0ea5b17