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Genetic and Functional Characterization of Toll-Like Receptor Responses in Immunocompetent Patients With CMV Mononucleosis
- Source :
- Frontiers in Cellular and Infection Microbiology, Vol 10 (2020), Frontiers in Cellular and Infection Microbiology
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Background: Human cytomegalovirus (CMV) modulates both innate and adaptive immune responses. However, limited data are available on the role of receptors of innate immunity, such as toll-like receptors (TLRs) in contributing to antiviral responses and inflammation. Objectives: The aim of this translational study was to characterize TLR responses in immunocompetent patients with primary and symptomatic CMV infection. Study Design: The study population consisted of 40 patients suffering from CMV mononucleosis and 124 blood donors included as controls. We evaluated the association between TLR2, 3, 4, 7 and 9 gene single nucleotide polymorphism (SNP) and susceptibility to symptomatic CMV infection in immunocompetent adults. Additionally, functional TLR-mediated cytokine responses in supernatants of short-term cultures of whole blood from patients with CMV mononucleosis and blood donors were evaluated. Results: TLR2 and TLR7/8 responses were altered in CMV infected patients as compared to healthy donors and were associated with the release of higher levels of the pro-inflammatory cytokines IL-6 and TNF-α, but not of the anti-inflammatory mediator IL-10. The analysis on the TLR SNPs indicated no difference between patients with CMV infection and the control group. Conclusions: No variation in the TLR2,3,4,7 and 9 genes was associated to the development of symptomatic CMV infection in immunocompetent adults. Nevertheless, TLR-mediated responses in CMV-infected patients appeared to be skewed toward a pro-inflammatory profile, which may contribute to the development of inflammatory symptoms during the CMV mononucleotic syndrome.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Human cytomegalovirus
Adult
Mononucleosis
cytomegaloviru
mononucleosis
medicine.medical_treatment
030106 microbiology
Immunology
lcsh:QR1-502
pro-inflammatory cytokines
Microbiology
lcsh:Microbiology
Proinflammatory cytokine
polymorphism
03 medical and health sciences
Cellular and Infection Microbiology
Immune system
medicine
Humans
cytomegalovirus
Toll-like receptor
Innate immune system
business.industry
virus diseases
Brief Research Report
medicine.disease
Immunity, Innate
TLR2
030104 developmental biology
Infectious Diseases
Cytokine
toll-like receptors
pro-inflammatory cytokine
Toll-Like Receptor 9
Cytomegalovirus Infections
Cytokines
mononucleosi
business
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular and Infection Microbiology
- Accession number :
- edsair.doi.dedup.....52a49ea72d53e23be1ec56e0794f99d1