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UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose
- Source :
- Fungal Genetics and Biology. 82:251-263
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- UDP-glucose pyrophosphorylase (UGP) is a key enzyme involved in carbohydrate metabolism, but there are few studies on the functions of this enzyme in fungi. The ugp gene of Ganoderma lucidum was cloned, and enzyme kinetic parameters of the UGP recombinant protein were determined in vitro, revealing that this protein was functional and catalyzed the reversible conversion between Glc-1-P and UDP-Glc. ugp silencing by RNA interference resulted in changes in the levels of the intermediate metabolites Glc-1-P and UDP-Glc. The compounds and structure of the cell wall in the silenced strains were also altered compared with those in the wild-type strains. Moreover, the number of hyphal branches was also changed in the silenced strains. To verify the role of UGP in hyphal branching, a ugp-overexpressing strain was constructed. The results showed that the number of hyphal branches was influenced by UGP. The mechanism underlying hyphal branching was further investigated by adding exogenous Glc-1-P. Our results showed that hyphal branching was regulated by a change in the cytosolic Ca(2+) concentration, which was affected by the level of the intermediate metabolite Glc-1-P, in G. lucidum. Our findings indicate the existence of an interaction between carbon metabolism and Ca(2+) signaling in this fungus.
- Subjects :
- Uridine Diphosphate Glucose
UTP-Glucose-1-Phosphate Uridylyltransferase
Hyphae
Glucose 1-phosphate
Carbohydrate metabolism
Biology
Polysaccharide
Microbiology
Cell wall
chemistry.chemical_compound
Enzyme activator
Cytosol
Cell Wall
Polysaccharides
Genetics
Cloning, Molecular
Phylogeny
chemistry.chemical_classification
UTP—glucose-1-phosphate uridylyltransferase
Basidiomycota
Glucosephosphates
Enzyme Activation
carbohydrates (lipids)
Enzyme
chemistry
Biochemistry
Mutation
Uridine diphosphate glucose
Calcium
RNA Interference
Subjects
Details
- ISSN :
- 10871845
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Fungal Genetics and Biology
- Accession number :
- edsair.doi.dedup.....52b25e3c55f09d766f3a009f9ae78c78