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Experimental Evidence for Membrane-Mediated Protein-Protein Interaction
- Source :
- Biophysical Journal, Biophysical Journal, Biophysical Society, 2010, 99 (7), pp.L47-L49. ⟨10.1016/j.bpj.2010.07.028⟩, Biophysical Journal, 2010, 99 (7), pp.L47-L49. ⟨10.1016/j.bpj.2010.07.028⟩
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Membrane proteins diffuse within the membrane, form oligomers and supramolecular assemblies. Using high-speed atomic force microscopy, we present direct experimental measure of an in-membrane-plane interaction potential between membrane proteins. In purple membranes, ATP-synthase c-rings formed dimers that temporarily dissociated. C-ring dimers revealed subdiffusive motion, while dissociated monomers diffused freely. C-rings center-to-center distance probability distribution allowed the calculation and modeling of an in-membrane-plane energy landscape that presented repulsion at 80 Å, most stable dimer association at 103 Å (−3.5 kBT strength), and dissociation at 125 Å (−1 kBT strength). This first experimental data of nonlabeled membrane protein diffusion and the corresponding in-membrane-plane interaction energy landscape characterized membrane protein interaction with an attractive range of several kBT that reaches to a radius of ∼50 Å within the membrane plane.
- Subjects :
- Halobacterium salinarum
Dimer
Biophysics
02 engineering and technology
Microscopy, Atomic Force
Dissociation (chemistry)
Protein–protein interaction
03 medical and health sciences
chemistry.chemical_compound
Purple Membrane
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
Biophysical Letter
Chemistry
Membrane Proteins
Energy landscape
Interaction energy
021001 nanoscience & nanotechnology
Crystallography
Monomer
Membrane
Membrane protein
Bacteriorhodopsins
[PHYS.COND.CM-GEN]Physics [physics]/Condensed Matter [cond-mat]/Other [cond-mat.other]
Thermodynamics
0210 nano-technology
Protein Binding
Subjects
Details
- ISSN :
- 00063495 and 15420086
- Volume :
- 99
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Biophysical Journal
- Accession number :
- edsair.doi.dedup.....52b692155bcada19009dfeb24e079d16
- Full Text :
- https://doi.org/10.1016/j.bpj.2010.07.028