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Analyte-Induced Desert Rose-like Ag Nanostructures for Surface-Enhanced Raman Scattering-Based Biomolecule Detection and Imaging
- Source :
- ACS Applied Materials & Interfaces. 13:58393-58400
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Biomolecule detection based on surface-enhanced Raman scattering (SERS) for application to biosensors and bio-imaging requires the fabrication of SERS nanoprobes that can generate strong Raman signals as well as surface modifications for analyte-specific recognition and binding. Such requirements lead to disadvantages in terms of reproducibility and practicality, and thus, it has been difficult to apply biomolecule detection utilizing the advantages of the SERS phenomenon to actual clinically relevant analysis. To achieve reproducible and practical SERS signal generation in a biomolecule-specific manner without requiring the synthesis of nanostructures and their related surface modification to introduce molecules for specific recognition, we developed a new type of SERS probe formed by enzyme reactions in the presence of Raman reporters. By forming unique plasmonic structures, our method achieves the detection of biomolecules on chips with uniform and stable signals over long periods. To test the proposed approach, we applied it to a SERS-based immunohistochemistry assay and found successful multiplexed protein detection in brain tissue from transgenic mice.
- Subjects :
- Analyte
Silver
Nanostructure
Materials science
Surface Properties
Metal Nanoparticles
Biocompatible Materials
Mice, Transgenic
Nanotechnology
Spectrum Analysis, Raman
Mice
symbols.namesake
Glial Fibrillary Acidic Protein
Materials Testing
Animals
General Materials Science
Particle Size
Plasmon
chemistry.chemical_classification
Amyloid beta-Peptides
Biomolecule
Brain
Actins
chemistry
symbols
Surface modification
Raman spectroscopy
Biosensor
Raman scattering
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....52c7e458647ccbdd05a77fdf42f72a77