Back to Search
Start Over
Identification of a novel germ cell marker MnTdrd from the oriental river prawn Macrobrachium nipponense
- Source :
- Development Genes and Evolution. 231:11-19
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Germ cell-specific genes play an important role in establishing the reproductive system in sexual organisms and have been used as valuable markers for studying gametogenesis and sex differentiation. Previously, we isolated a vasa transcript as a germ cell marker to trace the origin and migration of germ cells in the oriental river prawn Macrobrachium nipponense. Here, we identified a new germ cell-specific marker MnTdrd RNA and assessed its temporal and spatial expression during oogenesis and embryogenesis. MnTdrd transcripts were expressed in high abundance in unfertilized eggs and embryos at cleavage stage and then dropped significantly during late embryogenesis, suggesting that MnTdrd mRNA is maternally inherited. In situ hybridization of ovarian tissue showed that MnTdrd mRNA was initially present in the cytoplasm of previtellogenic oocyte and localized to the perinuclear region as the accumulation of yolk in vitellogenic oocyte. Whole-mount in situ hybridization of embryos showed that MnTdrd-positive signals were only localized in one blastomere until 16-cell stage. In the blastula, there were approximately 16 MnTdrd-positive blastomeres. During embryonized-zoea stage, the MnTdrd-positive cells aggregated as a cluster and migrated to the genital rudiment which would develop into primordial germ cells (PGCs). The localized expression pattern of MnTdrd transcripts resembled that of the previously identified germ cell marker vasa, supporting the preformation mode of germ cell specification. Therefore, we concluded that MnTdrd, together with vasa, is a component of the germ plasm and might have critical roles in germ cell formation and differentiation in the prawn. Thus, MnTdrd can be used as a novel germ cell marker to trace the origin and migration of germ cells.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Blastomeres
endocrine system
Cell Cycle Proteins
Biology
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
Genetics
medicine
Animals
Cell Lineage
Germ plasm
Tudor Domain
Embryo
Blastomere
Oocyte
Blastula
Cell biology
Germ Cells
030104 developmental biology
medicine.anatomical_structure
Oocytes
Vitellogenesis
Palaemonidae
Developmental biology
Germ cell
Developmental Biology
Subjects
Details
- ISSN :
- 1432041X and 0949944X
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Development Genes and Evolution
- Accession number :
- edsair.doi.dedup.....52da87c9b72647edb9bf9536cab76b85