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Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly

Authors :
Andreas Holzenburg
Stanislav Vitha
Aaron G. Smith
Carol B. Johnson
Source :
FEBS Letters. 584:166-172
Publication Year :
2009
Publisher :
Wiley, 2009.

Abstract

Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II filaments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca2+.

Details

ISSN :
00145793
Volume :
584
Database :
OpenAIRE
Journal :
FEBS Letters
Accession number :
edsair.doi.dedup.....52dbf2a8e928baaa281667f3765fefcd