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Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells

Authors :
Yanhong Yang
Weiyue Lu
Ningwen Zhu
Zihao Feng
Jianying Gu
Fazhi Qi
Jiaqi Liu
Source :
Journal of Translational Medicine
Publication Year :
2016
Publisher :
Springer Science and Business Media LLC, 2016.

Abstract

Background Histone deacetylase (HDAC) inhibitors are widely used in clinical investigation as novel drug targets. For example, panobinostat and vorinostat have been used to treat patients with melanoma. However, HDAC inhibitors are small-molecule compounds without a specific target, and their mechanism of action is unclear. Therefore, it is necessary to investigate which HDACs are required for the proliferation and metastasis of melanoma cells. Methods We used overexpression and knocking down lentivirus to clarify the influence of HDAC5 and HDAC6 in melanoma development. Also, we introduced stable HDAC5 or HDAC6 knockdown cells into null mice and found that the knockdown cells were unable to form solid tumors. Finally, we tested HDAC5 and HDAC6 expression and sub-location in clinical melanoma tissues and tumor adjacent tissues. Results In this study, and found that HDAC5 and HDAC6 were highly expressed in melanoma cells but exhibited low expression levels in normal skin cells. Furthermore, we knocked down HDAC5 or HDAC6 in A375 cells and demonstrated that both HDAC5 and HDAC6 contributed to the proliferation and metastasis of melanoma cells. Conclusions This study demonstrated both HDAC5 and HDAC6 were required for melanoma cell proliferation and metastasis through different signaling pathways. Electronic supplementary material The online version of this article (doi:10.1186/s12967-015-0753-0) contains supplementary material, which is available to authorized users.

Details

ISSN :
14795876
Volume :
14
Database :
OpenAIRE
Journal :
Journal of Translational Medicine
Accession number :
edsair.doi.dedup.....52de685006d0bbe73c467ea688af4fa0
Full Text :
https://doi.org/10.1186/s12967-015-0753-0