Serotoninergic denervation suppresses the sympathetic outflow induced by thyrotropin-releasing hormone in conscious rats

Intrathecal administration of thyrotropin-releasing hormone (TRH) resulted in an increase in plasma epinephrine (E) and glucose levels in conscious rats. To elucidate the mechanisms by which TRH stimulates the release of E, the animals were pretreated with 5,7-dihydroxytryptamine, parachlorophenylalanine, methysergide or ketanserin. Serotoninergic denervation suppressed the TRH-induced increase in plasma E and glucose levels, whereas inhibition of serotonin (5-HT) synthesis or blockade of 5-HT receptors did not suppress the responses. These findings suggest that the serotoninergic neurons, but not 5-HT itself, are involved in stimulating the sympathetic outflow by TRH at the spinal level.