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VF‐4 and DR‐8 Derived from Salted Egg White Inhibit Inflammatory Activity via NF‐κB/PI3K‐Akt/MAPK Signal Transduction Pathways in HT‐29 Cells Induced by TNF‐α
- Source :
- Molecular Nutrition & Food Research. 66:2100682
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Scope Inflammation is the pathological basis of many chronic diseases, and persistent intestinal inflammation is a key factor in the further development of colon cancer. Egg-derived peptides have been proven to have anti-intestinal inflammation activity. Egg white treated with salt contains a lot of rich protein, whether its peptides have anti-inflammatory activity and how their mechanism of action is still unclear. Methods and results In this study, ELISA was used to determine the anti-inflammatory activity of the peptides (VF-4 and DR-8 from salted egg white), and then RNA-seq was used to explore the mechanism of their anti-inflammatory activity, and then verified by western blotting and inhibitors. The results showed that VF-4 and DR-8 significantly inhibited TNF-α-induced IL-8 secretion in HT-29 cells in a concentration-dependent manner, and VF-4 showed a more significant anti-inflammatory effect than DR-8. The anti-inflammatory mechanism of VF-4 and DR-8 was through inhibiting the activation of NF-κB, MAPK, and PI3K-Akt pathways, reducing the production of inflammatory mediators. Conclusion VF-4 and DR-8 have obvious anti-inflammatory activity, which can reduce intestinal inflammation and inhibit its further development into colon cancer. This article is protected by copyright. All rights reserved.
- Subjects :
- Lipopolysaccharides
MAPK/ERK pathway
Tumor Necrosis Factor-alpha
Chemistry
NF-kappa B
Inflammation
NF-κB
Pharmacology
Blot
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
Egg White
Mechanism of action
medicine
Humans
Mitogen-Activated Protein Kinases
medicine.symptom
HT29 Cells
Proto-Oncogene Proteins c-akt
Protein kinase B
PI3K/AKT/mTOR pathway
Signal Transduction
Food Science
Biotechnology
Egg white
Subjects
Details
- ISSN :
- 16134133 and 16134125
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Molecular Nutrition & Food Research
- Accession number :
- edsair.doi.dedup.....52f516adcb9d7f5419f4b2cb335d007c