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Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development

Authors :
Dezene P. W. Huber
Christopher I. Keeling
Caitlin Pitt
Jeanne A. Robert
Tiffany R. Bonnett
Macaire M.S. Yuen
Jordie D. Fraser
Luke J. Spooner
Jörg Bohlmann
Source :
PeerJ, PeerJ, Vol 4, p e2109 (2016)
Publication Year :
2016
Publisher :
PeerJ, 2016.

Abstract

Cold-induced mortality has historically been a key aspect of mountain pine beetle,Dendroctonus ponderosaeHopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression patterns of mountain pine beetle larvae at four time points during their overwintering period—early-autumn, late-autumn, early-spring, and late-spring. Changing transcript profiles over the winter indicates a multipronged physiological response from larvae that is broadly characterized by gene transcripts involved in insect immune responses and detoxification during the autumn. In the spring, although transcripts associated with developmental process are present, there was no particular biological process dominating the transcriptome.

Details

ISSN :
21678359
Volume :
4
Database :
OpenAIRE
Journal :
PeerJ
Accession number :
edsair.doi.dedup.....53046a21ea963d5a29dfc4c5d8fd1ee7
Full Text :
https://doi.org/10.7717/peerj.2109