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Downregulation of miR-21 modulates Ras expression to promote apoptosis and suppress invasion of Laryngeal squamous cell carcinoma
- Source :
- European Journal of Cancer. 46:3409-3416
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- MiRNAs are small, noncoding RNA molecules that emerge as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally related to cellular proliferation and apoptosis. In this study, we found that miR-21 is overexpressed in Laryngeal squamous cell carcinoma (LSCC) and correlated with advanced stage. Inhibition of miR-21 by antisense oligonucleotides (ASO) led to decreased protein level of Ras and profound suppression of cell proliferation and invasion. Hep-2 cells exposed to miR-21 ASO exhibited cell cycle arrest at G1 phase and increased apoptosis. Furthermore, growth of LSCC xenograft tumours was significantly suppressed by repeated injection of ASO-miR-21 lentivirus and the Ras protein expression in LSCC xenograft tumours was also downregulate by ASO-miR-21. Taken together, our data suggest that miR-21 may play an oncogenic role in the cellular processes of LSCC and represent a novel target for effective therapies.
- Subjects :
- Male
Cancer Research
Programmed cell death
Cell cycle checkpoint
Down-Regulation
Apoptosis
Biology
Proto-Oncogene Proteins p21(ras)
Mice
Downregulation and upregulation
microRNA
Tumor Cells, Cultured
medicine
Animals
Humans
Neoplasm Invasiveness
Laryngeal Neoplasms
Mice, Inbred BALB C
Cell growth
Cancer
Middle Aged
medicine.disease
Non-coding RNA
Neoplasm Proteins
MicroRNAs
Oncology
Immunology
Carcinoma, Squamous Cell
Cancer research
Female
Subjects
Details
- ISSN :
- 09598049
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- European Journal of Cancer
- Accession number :
- edsair.doi.dedup.....530c74fa9ec554c87262b1fc18279c66