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Tunable keratin hydrogel based on disulfide shuffling strategy for drug delivery and tissue engineering
- Source :
- Journal of colloid and interface science. 544
- Publication Year :
- 2018
-
Abstract
- Protein-based hydrogels that possess tunable properties have long been a challenge in tissue engineering. Keratin is a group of natural proteins derived from skin and skin appendant, and features a rich content of cysteine residue which exists in the form of disulfide bonds. Inspired by this, in this work, a simple disulfide shuffling strategy was utilized to develop keratin hydrogels by converting the intramolecular disulfide bonds into the intermolecular disulfide bonds. To achieve this, the intramolecular disulfide bonds were first cleaved by the reductive reagent such as cysteine, to liberate free thiol group, which formed intermolecular disulfide bonds through thiol oxidation. It was demonstrated that control of the cysteine level led to a tunable disulfide crosslinking density, and thus an altered network structure, gel degradation, and drug release rate. Also, this strategy enables good biocompatibility of the material owing to avoiding extra chemical crosslinkers in the preparation procedure. Moreover, this keratin hydrogel had redox-responsive capacity in both gel degradation and drug release due to the disulfide-bond based network structure, providing extensive applicability in tissue engineering and drug release.
- Subjects :
- Biocompatibility
Cell Survival
Biocompatible Materials
macromolecular substances
02 engineering and technology
010402 general chemistry
01 natural sciences
Cell Line
Biomaterials
Residue (chemistry)
Mice
Structure-Activity Relationship
Colloid and Surface Chemistry
Keratin
Materials Testing
Animals
Cysteine
Disulfides
chemistry.chemical_classification
Drug Carriers
Mice, Inbred ICR
Molecular Structure
Tissue Engineering
Tissue Scaffolds
Chemistry
Hydrogels
021001 nanoscience & nanotechnology
Combinatorial chemistry
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Rats
Drug Liberation
Cross-Linking Reagents
Intramolecular force
Reagent
Drug delivery
Self-healing hydrogels
Keratins
0210 nano-technology
Rheology
Oxidation-Reduction
Subjects
Details
- ISSN :
- 10957103
- Volume :
- 544
- Database :
- OpenAIRE
- Journal :
- Journal of colloid and interface science
- Accession number :
- edsair.doi.dedup.....530d50da009c86a703349bee748a3fb3