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Identification of suitable reference genes for studies of Syringa pinnatifolia Hemsl

Authors :
Suyile Chen
Luqi Huang
Chao Jiang
Juan Liu
Jiaqi Gao
Source :
FEBS Open Bio, Vol 11, Iss 4, Pp 1041-1053 (2021), FEBS Open Bio
Publication Year :
2021
Publisher :
Wiley, 2021.

Abstract

Syringa pinnatifolia Hemsl. (Oleaceae) is a species of shrub with a limited distribution in China. Several chemical compounds with pharmacological effects have been isolated from S. pinnatifolia, including new lignans and sesquiterpenes. Studies of gene expression in this species require the identification of suitable reference genes that are stably expressed under different conditions and in different tissues. To identify candidate reference genes, here we used the geNorm, NormFinder, and BestKeeper algorithms to analyze the stability of 12 candidate genes. The geometric mean of the rankings generated with these algorithms was used to obtain a comprehensive ranking. TBP and PP2A were found to be appropriate reference genes for calli treated with different external stimuli, and TIP41 and TBP were found to be appropriate reference genes in differentiated tissues. When calli and differentiated tissues were considered together, TBP and TIP41 were found to be the most reliable reference genes. The selected genes were validated by analysis of HMGR expression in calli and differentiated tissues. This study is the first to screen candidate reference genes in the genus Syringa and could help guide future molecular studies in this genus.<br />Appropriate and stable reference genes are important for normalization of gene expression. Here, we estimated the stability of 12 candidate reference genes in Syringa pinnatifolia. This study is the first to screen candidate reference genes in the genus Syringa and could help guide future molecular studies in this genus.

Details

ISSN :
22115463
Volume :
11
Database :
OpenAIRE
Journal :
FEBS Open Bio
Accession number :
edsair.doi.dedup.....5333504c55d26f04dea1c4a6e9ccab5b
Full Text :
https://doi.org/10.1002/2211-5463.13097