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Identification of suitable reference genes for studies of Syringa pinnatifolia Hemsl
- Source :
- FEBS Open Bio, Vol 11, Iss 4, Pp 1041-1053 (2021), FEBS Open Bio
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Syringa pinnatifolia Hemsl. (Oleaceae) is a species of shrub with a limited distribution in China. Several chemical compounds with pharmacological effects have been isolated from S. pinnatifolia, including new lignans and sesquiterpenes. Studies of gene expression in this species require the identification of suitable reference genes that are stably expressed under different conditions and in different tissues. To identify candidate reference genes, here we used the geNorm, NormFinder, and BestKeeper algorithms to analyze the stability of 12 candidate genes. The geometric mean of the rankings generated with these algorithms was used to obtain a comprehensive ranking. TBP and PP2A were found to be appropriate reference genes for calli treated with different external stimuli, and TIP41 and TBP were found to be appropriate reference genes in differentiated tissues. When calli and differentiated tissues were considered together, TBP and TIP41 were found to be the most reliable reference genes. The selected genes were validated by analysis of HMGR expression in calli and differentiated tissues. This study is the first to screen candidate reference genes in the genus Syringa and could help guide future molecular studies in this genus.<br />Appropriate and stable reference genes are important for normalization of gene expression. Here, we estimated the stability of 12 candidate reference genes in Syringa pinnatifolia. This study is the first to screen candidate reference genes in the genus Syringa and could help guide future molecular studies in this genus.
- Subjects :
- 0301 basic medicine
Candidate gene
RNA Stability
reference gene
Computational biology
Genes, Plant
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Quantitative Trait, Heritable
0302 clinical medicine
Gene Expression Regulation, Plant
Reference genes
Gene expression
lcsh:QH301-705.5
Gene
Research Articles
Plant Physiological Phenomena
biology
Gene Expression Profiling
Computational Biology
Molecular Sequence Annotation
qRT‐PCR
Syringa
Syringa pinnatifolia
biology.organism_classification
030104 developmental biology
Real-time polymerase chain reaction
lcsh:Biology (General)
030220 oncology & carcinogenesis
Oleaceae
gene expression
Identification (biology)
Syringa pinnatifolia Hemsl
Research Article
Subjects
Details
- ISSN :
- 22115463
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- FEBS Open Bio
- Accession number :
- edsair.doi.dedup.....5333504c55d26f04dea1c4a6e9ccab5b
- Full Text :
- https://doi.org/10.1002/2211-5463.13097