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Ammonium stress increases microautophagic activity while impairing macroautophagic flux in Arabidopsis roots
- Source :
- Plant Journal, Plant Journal, Wiley, 2021, 105 (4), pp.1083-1097. ⟨10.1111/tpj.15091⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Plant responses to NH4 (+) stress are complex, and multiple mechanisms underlying NH4 (+) sensitivity and tolerance in plants may be involved. Here, we demonstrate that macro- and microautophagic activities are oppositely affected in plants grown under NH4 (+) toxicity conditions. When grown under NH4 (+) stress conditions, macroautophagic activity was impaired in roots. Root cells accumulated autophagosomes in the cytoplasm, but showed less autophagic flux, indicating that late steps of the macroautophagy process are affected under NH4 (+) stress conditions. Under this scenario, we also found that the CCZ1-MON1 complex, a critical factor for vacuole delivery pathways, functions in the late step of the macroautophagic pathway in Arabidopsis. In contrast, an accumulation of tonoplast-derived vesicles was observed in vacuolar lumens of root cells of NH4 (+)-stressed plants, suggesting the induction of a microautophagy-like process. In this sense, some SYP22-, but mainly VAMP711-positive vesicles were observed inside vacuole in roots of NH4 (+)-stressed plants. Consistent with the increased tonoplast degradation and the reduced membrane flow to the vacuole due to the impaired macroautophagic flux, the vacuoles of root cells of NH4 (+)-stressed plants showed a simplified structure and lower tonoplast content. Taken together, this study presents evidence that postulates late steps of the macroautophagic process as a relevant physiological mechanism underlying the NH4 (+) sensitivity response in Arabidopsis, and additionally provides insights into the molecular tools for studying microautophagy in plants.
- Subjects :
- 0106 biological sciences
0301 basic medicine
inorganic chemicals
microautophagy
Arabidopsis
CCZ1‐
Plant Science
Vacuole
Plant Roots
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Stress, Physiological
Ammonium Compounds
Genetics
vacuole fusion
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Ammonium
Microautophagy
NH4 (+) toxicity
biology
Vesicle
Autophagy
vacuole morphology
Autophagosomes
food and beverages
Cell Biology
biology.organism_classification
to‐
macroautophagy
030104 developmental biology
chemistry
Cytoplasm
Biophysics
MON1
Flux (metabolism)
autophagosome‐
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 09607412 and 1365313X
- Database :
- OpenAIRE
- Journal :
- Plant Journal, Plant Journal, Wiley, 2021, 105 (4), pp.1083-1097. ⟨10.1111/tpj.15091⟩
- Accession number :
- edsair.doi.dedup.....5358fab385f34c747a2473bcdbdd4b6d