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Survivin as a Prognostic Factor for Osteosarcoma Patients
- Source :
- Acta Histochemica et Cytochemica
- Publication Year :
- 2006
- Publisher :
- Japan Society of Histochemistry & Cytochemistry, 2006.
-
Abstract
- Purpose: Survivin is one of the apoptosis inhibitor genes and is rarely expressed in adult ­tissues. However, survivin expression has been detected in various human cancers and ­correlations have been recognized between the level of expression of this gene in tumors and prognosis. In this study, we investigated the correlations between survivin mRNA expres­sion in osteosarcoma tissues and clinicopathological parameters. Methods: There were 22 osteosarcoma patients in our hospital with paraffin-embedded ­tissues which could be extracted from biopsy specimens. We used the RT-PCR method after extracting total RNA and conducted a densitometric analysis to determine the ratio of survivin relative to h-GAPDH as an internal marker. Results: Expression of survivin mRNA was detected in all osteosarcoma samples. Patients with metastasis had high survivin mRNA levels in initial biopsy specimens (p
- Subjects :
- Prognostic factor
Pathology
medicine.medical_specialty
Histology
Apoptosis Inhibitor
Physiology
survivin
Biochemistry
Pathology and Forensic Medicine
Metastasis
osteosarcoma
Survivin
Biopsy
medicine
prognostic factor
neoplasms
Gene
Messenger RNA
medicine.diagnostic_test
business.industry
Regular Article
Cell Biology
medicine.disease
Cancer research
Osteosarcoma
paraffin-embedded tissue
business
Subjects
Details
- ISSN :
- 13475800 and 00445991
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- ACTA HISTOCHEMICA ET CYTOCHEMICA
- Accession number :
- edsair.doi.dedup.....535be59f5ba8bf8778f61d51239dcbf3