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Iron uptake mediated by the plant-derived chelator nicotianamine in the small intestine
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2021
- Publisher :
- American Society for Biochemistry & Molecular Biology (ASBMB), 2021.
-
Abstract
- Iron is an essential metal for all living organisms that is absorbed in the intestinal cells as a heme-chelated or free form. It is unclear how important plant-derived chelators, such as nicotianamine (NA), an organic small molecule that is ubiquitous in crops, vegetables, and various other foods, contribute to iron bioavailability in mammals. We performed electrophysiological assays with Xenopus laevis oocytes and radioactive tracer experiments with Caco-2 cells. The findings revealed that the proton-coupled amino acid transporter SLC36A1 (PAT1) transports iron in the form of NA-Fe (II) complex in vitro. Decreased expression of hPAT1 by RNA interference in Caco-2 cells reduced the uptake of NA-59Fe (II) complex. The uptake of inorganic 59Fe (II) was relatively unaffected. These results imply that PAT1 transports iron as a NA-Fe (II) complex. The rate of 59Fe absorption in the spleen, liver, and kidney was higher when mice were orally administered NA-59Fe (II) compared with free 59Fe (II). The profile of site-specific PAT1 expression in the mouse intestine coincided with those of NA and iron contents, which were the highest in the proximal jejunum. Orally administered NA-59Fe (II) complex in mice was detected in the proximal jejunum by thin layer chromatography. In contrast, much less 59Fe (or NA) was detected in the duodenum, where the divalent metal transporter SLC11A2 (DMT1) absorbs free Fe (II). The collective results revealed the role of PAT1 in NA-Fe (II) absorption in the intestine and potential implication of NA in iron uptake in mammals.
- Subjects :
- Male
0301 basic medicine
5-HTP, 5-hydroxytryptophan
Xenopus
Phytochemicals
Caco-2 cells
FMOC, 9-fluorenylmethyloxycarbonyl
Biochemistry
Mice
Xenopus laevis
chemistry.chemical_compound
DMT1, divalent metal transporter
Intestine, Small
Nicotianamine
Cells, Cultured
Chelating Agents
Mice, Inbred ICR
SLC36A1
biology
Chemistry
plant-derived chelator
NA, nicotianamine
transport metal
Jejunum
medicine.anatomical_structure
transporter
Azetidinecarboxylic Acid
Research Article
Duodenum
Iron
Biological Availability
MAs, phytosiderophores
Dcytb, duodenal cytochrome b
PEPT1, oligopeptide transporter
03 medical and health sciences
Duodenal cytochrome B
medicine
Animals
Humans
Amino acid transporter
oocyte
intestine
Molecular Biology
TLC, thin layer chromatography
030102 biochemistry & molecular biology
PAT1, proton-coupled amino acid transporter
nicotianamine
Biological Transport
Cell Biology
DMT1
Small intestine
Bioavailability
FPN1, ferroportin1
030104 developmental biology
Intestinal Absorption
biology.protein
YS1/YSL, yellow stripe 1/yellow stripe like
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 296
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....5360521e40b094df7faf8aee1fb2d3bd