Back to Search
Start Over
Genotypic distribution of common variants of endosomal toll like receptors in healthy Spanish women. A comparative study with other populations
- Source :
- Gene. 578(1)
- Publication Year :
- 2015
-
Abstract
- Genetic variants of endosomal toll like receptors (TLR) have been associated with many infectious, autoimmune and inflammatory diseases, but few studies have been reported in the Spanish population. The aim of this study was to describe the allelic and genotypic distributions of some common nucleotide substitutions of endosomal TLRs in healthy Spanish women and to compare them with those already published in other population groups. Nine substitutions were analysed in 150 DNA samples from 150 Spanish, non-related healthy females: TLR3 rs3775291 and rs5743305; TLR7 rs179008 and rs5743781; TLR8 rs3764880 and TLR9 rs187084, rs5743836, rs352139 and rs352140. Genotyping was carried out by real time PCR and melting curve analysis in a LightCycler 480. A systematic review was performed in order to compare the genotypic distributions in our cohort with those previously published in other population groups. The comparative study was performed with the two tailed Fisher's test or the Yates continuity correction for the Chi-square test when appropriate. No homozygotes for rs5743781 in TLR7 were found, and rs352139 and rs352140 of TLR9 were in strong linkage disequilibrium. Genotype distributions in endosomal TLR are similar to other Spanish series previously reported. As expected, most differences were found when comparing our distributions with Asiatics, but differences were also found with other Caucasian populations. Since there are significant variations in genotypic distributions of TLRs in both interracial groups and within the same ethnic group, to carry out studies of disease susceptibility in more restricted groups is mandatory.
- Subjects :
- 0301 basic medicine
Adult
Linkage disequilibrium
Population
Biology
White People
03 medical and health sciences
Young Adult
0302 clinical medicine
Asian People
Genotype
Genetics
Humans
Allele
education
Yates's correction for continuity
Genotyping
030203 arthritis & rheumatology
education.field_of_study
Toll-Like Receptors
General Medicine
Healthy Volunteers
Toll-Like Receptor 3
030104 developmental biology
Real-time polymerase chain reaction
Amino Acid Substitution
Toll-Like Receptor 7
Spain
Toll-Like Receptor 8
Toll-Like Receptor 9
Cohort
Female
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 578
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....5378bcaa2ca10e64299f6fe60476de38