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Multimodal highly fluorescent-magnetic nanoplatform to target transferrin receptors in cancer cells
- Source :
- Biochimica et Biophysica Acta (BBA) - General Subjects. 1862:2788-2796
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Background Site-specific multimodal nanoplatforms with fluorescent-magnetic properties have great potential for biological sciences. For this reason, we developed a multimodal nanoprobe (BNPs-Tf), by covalently conjugating an optical-magnetically active bimodal nanosystem, based on quantum dots and iron oxide nanoparticles, with the human holo-transferrin (Tf). Methods The Tf bioconjugation efficiency was evaluated by the fluorescence microplate assay (FMA) and the amount of Tf immobilized on BNPs was quantified by fluorescence spectroscopy. Moreover, relaxometric and fluorescent properties of the BNPs-Tf were evaluated, as well as its ability to label specifically HeLa cells. Cytotoxicity was also performed by Alamar Blue assay. Results The FMA confirmed an efficient bioconjugation and the fluorescence spectroscopy analysis indicated that 98% of Tf was immobilized on BNPs. BNPs-Tf also presented a bright fluorescence and a transversal/longitudinal relaxivities ratio (r2/r1) of 65. Importantly, the developed BNPs-Tf were able to label, efficiently and specifically, the Tf receptors in HeLa cells, as shown by fluorescence and magnetic resonance imaging assays. Moreover, this multimodal system did not cause noteworthy cytotoxicity. Conclusions The prepared BNPs-Tf hold great promise as an effective and specific multimodal, highly fluorescent-magnetic, nanoplatform for fluorescence analyses and T2-weighted images. General significance This study developed an attractive and versatile multimodal nanoplatform that has potential to be applied in a variety of in vitro and in vivo studies, addressing biological processes, diagnostic, and therapeutics. Moreover, this work opens new possibilities for designing other efficient multimodal nanosystems, considering other biomolecules in their composition able to provide them important functional properties.
- Subjects :
- Biophysics
Nanoprobe
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Fluorescence spectroscopy
HeLa
Magnetics
chemistry.chemical_compound
Quantum Dots
Receptors, Transferrin
Humans
Cytotoxicity
Molecular Biology
Fluorescent Dyes
chemistry.chemical_classification
Bioconjugation
biology
Biomolecule
Transferrin
Flow Cytometry
021001 nanoscience & nanotechnology
biology.organism_classification
Magnetic Resonance Imaging
Fluorescence
0104 chemical sciences
Spectrometry, Fluorescence
chemistry
Nanoparticles
0210 nano-technology
Iron oxide nanoparticles
HeLa Cells
Subjects
Details
- ISSN :
- 03044165
- Volume :
- 1862
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Accession number :
- edsair.doi.dedup.....53e6a556e46bd55ae5fa75e4d885d6be
- Full Text :
- https://doi.org/10.1016/j.bbagen.2018.08.014