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Evaluation of Carbohydrates in Natural and Cultured Cordyceps by Pressurized Liquid Extraction and Gas Chromatography Coupled with Mass Spectrometry
- Source :
- Molecules, Molecules, Vol 15, Iss 6, Pp 4227-4241 (2010), Molecules; Volume 15; Issue 6; Pages: 4227-4241
- Publication Year :
- 2010
- Publisher :
- MDPI, 2010.
-
Abstract
- Free and polymeric carbohydrates in Cordyceps, a valued edible mushroom and well-known traditional Chinese medicine, were determined using stepwise pressurized liquid extraction (PLE) extraction and GC-MS. Based on the optimized PLE conditions, acid hydrolysis and derivatization, ten monosaccharides, namely rhamnose, ribose, arabinose, xylose, mannose, glucose, galactose, mannitol, fructose and sorbose in 13 samples of natural and cultured Cordyceps were qualitatively and quantitatively analyzed and compared with myo-inositol hexaacetate as internal standard. The results showed that natural C. sinensis contained more than 7.99% free mannitol and a small amount of glucose, while its polysaccharides were usually composed of mannose, glucose and galactose with a molar ratio of 1.00:16.61~3.82:1.60~1.28. However, mannitol in cultured C. sinensis and cultured C. militaris were less than 5.83%, and free glucose was only detected in a few samples, while their polysaccharides were mainly composed of mannose, glucose and galactose with molar ratios of 1.00:3.01~1.09:3.30~1.05 and 1.00:2.86~1.28:1.07~0.78, respectively. Natural and cultured Cordyceps could be discriminated by hierarchical clustering analysis based on its free carbohydrate contents.
- Subjects :
- polysaccharides
Carbohydrates
Pharmaceutical Science
Mannose
Polysaccharide
Article
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
lcsh:QD241-441
chemistry.chemical_compound
lcsh:Organic chemistry
pressurized liquid extraction (PLE)
Drug Discovery
Monosaccharide
GC–MS
Physical and Theoretical Chemistry
chemistry.chemical_classification
Cordyceps
Chromatography
biology
carbohydrates
Organic Chemistry
Fructose
Carbohydrate
Sorbose
biology.organism_classification
chemistry
Chemistry (miscellaneous)
Galactose
Molecular Medicine
GC-MS
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....53ebf3b0dc4de71d30fb183d929b07fb