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Enhancement of insulin-mediated rat muscle glucose uptake and microvascular perfusion by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside

Enhancement of insulin-mediated rat muscle glucose uptake and microvascular perfusion by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside

Authors :
Lei Zhang
Stephen Rattigan
Amanda J Genders
Eloise A. Bradley
Stephen M. Richards
Michelle A. Keske
Source :
Cardiovascular Diabetology
Publisher :
Springer Nature

Abstract

Background Insulin-induced microvascular recruitment is important for optimal muscle glucose uptake. 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR, an activator of AMP-activated protein kinase), can also induce microvascular recruitment, at doses that do not acutely activate glucose transport in rat muscle. Whether low doses of AICAR can augment physiologic insulin action is unknown. In the present study we used the euglycemic hyperinsulinemic clamp to assess whether insulin action is augmented by low dose AICAR. Methods Anesthetized rats were studied during saline infusion or euglycemic insulin (3 mU/kg/min) clamp for 2 h in the absence or presence of AICAR for the last hour (5 mg bolus followed by 3.75 mg/kg/min). Muscle glucose uptake (R’g) was determined radioisotopically with 14C-2-deoxyglucose and muscle microvascular perfusion by contrast-enhanced ultrasound with microbubbles. Results AICAR did not affect blood glucose, or lower leg R’g, although it significantly (p

Details

Language :
English
ISSN :
14752840
Volume :
14
Issue :
1
Database :
OpenAIRE
Journal :
Cardiovascular Diabetology
Accession number :
edsair.doi.dedup.....53f1b0d7a8dd962106cf624b2b8be47b
Full Text :
https://doi.org/10.1186/s12933-015-0251-y