Back to Search
Start Over
Enhancement of insulin-mediated rat muscle glucose uptake and microvascular perfusion by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside
Enhancement of insulin-mediated rat muscle glucose uptake and microvascular perfusion by 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside
- Source :
- Cardiovascular Diabetology
- Publisher :
- Springer Nature
-
Abstract
- Background Insulin-induced microvascular recruitment is important for optimal muscle glucose uptake. 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR, an activator of AMP-activated protein kinase), can also induce microvascular recruitment, at doses that do not acutely activate glucose transport in rat muscle. Whether low doses of AICAR can augment physiologic insulin action is unknown. In the present study we used the euglycemic hyperinsulinemic clamp to assess whether insulin action is augmented by low dose AICAR. Methods Anesthetized rats were studied during saline infusion or euglycemic insulin (3 mU/kg/min) clamp for 2 h in the absence or presence of AICAR for the last hour (5 mg bolus followed by 3.75 mg/kg/min). Muscle glucose uptake (R’g) was determined radioisotopically with 14C-2-deoxyglucose and muscle microvascular perfusion by contrast-enhanced ultrasound with microbubbles. Results AICAR did not affect blood glucose, or lower leg R’g, although it significantly (p
- Subjects :
- Male
medicine.medical_specialty
Time Factors
medicine.medical_treatment
Glucose uptake
Endocrinology, Diabetes and Metabolism
Contrast Media
Blood volume
Deoxyglucose
Microcirculation
Diabetes mellitus
Internal medicine
medicine
Animals
Hypoglycemic Agents
Insulin
Lactic Acid
Rats, Wistar
Muscle, Skeletal
Ultrasonography
Original Investigation
Microbubbles
biology
business.industry
Glucose transporter
Ribonucleotides
Glucose clamp technique
Aminoimidazole Carboxamide
medicine.disease
Hindlimb
Femoral Artery
Nitric oxide synthase
Muscle Fibers, Slow-Twitch
Glucose
Endocrinology
Regional Blood Flow
Microvessels
Muscle Fibers, Fast-Twitch
Glucose Clamp Technique
biology.protein
Muscle
Nitric Oxide Synthase
business
Cardiology and Cardiovascular Medicine
Blood Flow Velocity
Subjects
Details
- Language :
- English
- ISSN :
- 14752840
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cardiovascular Diabetology
- Accession number :
- edsair.doi.dedup.....53f1b0d7a8dd962106cf624b2b8be47b
- Full Text :
- https://doi.org/10.1186/s12933-015-0251-y