Back to Search
Start Over
BMP-2 and VEGF-A modRNAs in collagen scaffold synergistically drive bone repair through osteogenic and angiogenic pathways
- Source :
- Communications Biology, Vol 4, Iss 1, Pp 1-14 (2021), Communications Biology
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Bone has a remarkable potential for self-healing and repair, yet several injury types are non-healing even after surgical or non-surgical treatment. Regenerative therapies that induce bone repair or improve the rate of recovery are being intensely investigated. Here, we probed the potential of bone marrow stem cells (BMSCs) engineered with chemically modified mRNAs (modRNA) encoding the hBMP-2 and VEGF-A gene to therapeutically heal bone. Induction of osteogenesis from modRNA-treated BMSCs was confirmed by expression profiles of osteogenic related markers and the presence of mineralization deposits. To test for therapeutic efficacy, a collagen scaffold inoculated with modRNA-treated BMSCs was explored in an in vivo skull defect model. We show that hBMP-2 and VEGF-A modRNAs synergistically drive osteogenic and angiogenic programs resulting in superior healing properties. This study exploits chemically modified mRNAs, together with biomaterials, as a potential approach for the clinical treatment of bone injury and defects.<br />Geng et al. evaluate bone marrow stem cell (BMSC)-based system to deliver modified RNAs of BMP-2 and VEGF to enhance bone regeneration. They test its therapeutic efficacy in vivo on a rat skull defect model by inoculating these BMSCs in a collagen scaffold. This construct synergistically drives osteogenic and angiogenic pathways and can be a new approach for clinical treatment of bone injuries and defects.
- Subjects :
- Male
Vascular Endothelial Growth Factor A
0301 basic medicine
China
Bone Regeneration
QH301-705.5
Cellular differentiation
Bone Morphogenetic Protein 2
Neovascularization, Physiologic
Stem-cell differentiation
Medicine (miscellaneous)
Biocompatible Materials
Bone Marrow Cells
02 engineering and technology
Bone healing
Bone morphogenetic protein 2
Bone and Bones
Article
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
03 medical and health sciences
Tissue engineering
Osteogenesis
In vivo
Animals
RNA, Messenger
Biology (General)
Bone
Bone regeneration
Cells, Cultured
Tissue Engineering
Bone Injury
Chemistry
Bone Marrow Stem Cell
Cell Differentiation
Mesenchymal Stem Cells
Translational research
021001 nanoscience & nanotechnology
Rats
030104 developmental biology
Cancer research
Collagen
0210 nano-technology
General Agricultural and Biological Sciences
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 4
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....5401a4850539f01a9f330c60409177c9