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Periostin down-regulation attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF-β1
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2015
-
Abstract
- Liver fibrosis is characterized by an exacerbated accumulation of deposition of the extracellular matrix (ECM), and the activation of hepatic stellate cells (HSC) plays a pivotal role in the development of liver fibrosis. Periostin has been shown to regulate cell adhesion, proliferation, migration and apoptosis; however, the involvement of periostin and its role in transforming growth factor (TGF)-β1-induced HSC activation remains unclear. We used RT-PCR and Western blot to evaluate the expression level of periostin in hepatic fibrosis tissues and HSCs, respectively. Cell proliferation was determined using the Cell Proliferation ELISA BrdU kit, cell cycle was analysed by flow cytometry. The expression of α-smooth muscle actin (α-SMA), collagen I, TGF-β1, p-Smad2 and p-Smad3 were determined by western blot. Our study found that periostin was up-regulated in liver fibrotic tissues and activated HSCs. In addition, siRNA-periostin suppressed TGF-β1-induced HSC proliferation. The HSC transfected with siRNA-periostin significantly inhibited TGF-β1-induced expression levels of α-SMA and collagen I. Furthermore, TGF-β1 stimulated the expression of periostin, and siRNA-periostin attenuated TGF-β1-induced Smad2/3 activation in HSCs. These results suggest that periostin may function as a novel regulator to modulate HSC activation, potentially by promoting the TGF-β1/Smad signalling pathway, and propose a strategy to target periostin for the treatment of liver fibrosis.
- Subjects :
- Liver Cirrhosis
Pathology
medicine.medical_specialty
Down-Regulation
SMAD
Periostin
Biology
Collagen Type I
Extracellular matrix
Transforming Growth Factor beta1
Fibrosis
TGF-β1
medicine
Hepatic Stellate Cells
Humans
RNA, Small Interfering
Cell Proliferation
periostin
Cell growth
Cell adhesion molecule
Cell Cycle
fibrosis
Cell Biology
Original Articles
medicine.disease
Actins
Cell biology
Up-Regulation
Hepatic stellate cell
Molecular Medicine
Hepatic fibrosis
Cell Adhesion Molecules
Signal Transduction
Subjects
Details
- ISSN :
- 15824934
- Volume :
- 19
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of cellular and molecular medicine
- Accession number :
- edsair.doi.dedup.....54037dad52e3211fa0f783e34e50a8f9