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Pocket Epithelium in the Pathological Setting for HMGB1 Release
- Source :
- Journal of Dental Research. 90:235-240
- Publication Year :
- 2010
- Publisher :
- SAGE Publications, 2010.
-
Abstract
- High-mobility group box-1 (HMGB1) protein acts as a transcription factor in the nucleus and also as a pro-inflammatory cytokine when released into extracellular fluids. The presence of higher levels of HMGB1 is reported in the gingival crevicular fluid from periodontal patients. Since the proliferation of bacteria within the periodontal pocket is closely involved in the exacerbation of periodontal disease, it is hypothesized that the periodontal pocket causes the release of HMGB1. Immunohistochemical staining of inflamed gingiva revealed that HMGB1 is exclusively dislocated from the nucleus to the cytoplasm in the pocket epithelium, whereas it is mainly present in the nucleus in the gingival epithelium. Butyric acid, an extracellular metabolite from periodontopathic bacteria populating the periodontal pocket, induced the passive release of HMGB1 as a result of eliciting necrosis in the human gingival epithelial cell line. Thus, the periodontal epithelium may provide a unique pathological setting for HMGB1 release by bacterial insult. Abbreviations: HMGB1, high-mobility group box-1; TNF-α, tumor necrosis factor-α; CHX, cycloheximide; PI, propidium iodide; ROS, reactive oxygen species; HDAC, histone deacetylase.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Necrosis
Gingival and periodontal pocket
Virulence Factors
medicine.medical_treatment
Gingiva
Junctional epithelium
chemical and pharmacologic phenomena
HMGB1
Cell Line
medicine
Humans
Periodontal Pocket
HMGB1 Protein
General Dentistry
Aged
biology
Epithelial Cells
Gingival Crevicular Fluid
Middle Aged
Epithelium
Protein Transport
medicine.anatomical_structure
Cytokine
Clinical attachment loss
Cytoplasm
Case-Control Studies
biology.protein
Butyric Acid
Female
medicine.symptom
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 15440591 and 00220345
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Journal of Dental Research
- Accession number :
- edsair.doi.dedup.....54152dbe5eabf524279774323bc1e5f5